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pCMV-dCas12a-A3A-N57G

Plasmid

#193639

Purpose

Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y130F

Plasmid

#193640

Purpose

Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo-YAP

Plasmid

#166468

Purpose

Plasmid coding for YAP and neomycin resistance in a lentiviral backbone.

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP, Neo (YAP1 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NES-YFP-CCTc

Plasmid

#184328

Purpose

Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.2)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTc (CACNA1C Human)

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-v2-gYap-1

Plasmid

#166481

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-TNT-CFP-STIM1-C227W

Plasmid

#68403

Purpose

Expresses a STIM1 gain-of-function mutant in mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

CFP-STIM1 (STIM1 Human)

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Mutation

Changed Cysteine 227 to Tryptophan; CFP was inser…

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

Plasmid

#204852

Purpose

AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSaCas9KKH(D10A)-UGI

Use

AAV and CRISPR

Mutation

D10A, E782K, N968K, R1015H in SaCas9

Promoter

EFS, miniU6

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A-deltaCC

Plasmid

#166459

Purpose

Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltaCC (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-V2-gYap-2

Plasmid

#166482

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-NF2

Plasmid

#166473

Purpose

Expresses fusion of mEGFP and NF2

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, NF2 (NF2 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L308E

Plasmid

#166462

Purpose

Expresses fusion of mEGFP and YAPS127A- L308E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L308E (YAP1 Human)

Expression

Mammalian

Mutation

YAPS127A- L308E

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A-deltasIDR

Plasmid

#166460

Purpose

Expresses fusion of mEGFP and YAPdeltasIDR (delta360-449)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltasIDR (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A-deltasTAD

Plasmid

#166461

Purpose

Expresses fusion of mEGFP and YAPdeltasTAD (delta450-504)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltasTAD (YAP1 Human)

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-coiled coil

Plasmid

#166448

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, coiled coil(298-359) (CRY2 Human)

Use

Lentiviral

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L325E

Plasmid

#166464

Purpose

Expresses fusion of mEGFP and YAPS127A- L325E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L325E (YAP1 Human)

Expression

Mammalian

Mutation

YAPS127A- L325E

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NES-YFP-CCTd

Plasmid

#184326

Purpose

Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.3)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTd (CACNA1D Human)

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NLS-YFP-CCTd

Plasmid

#184325

Purpose

Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTd (CACNA1D Human)

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L318E

Plasmid

#166465

Purpose

Expresses fusion of mEGFP and YAPS127A- L318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L318E (YAP1 Human)

Expression

Mammalian

Mutation

YAPS127A- L318E

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+Brd9 (zebrafish) partial UTR and coding sequence-EGFP

Plasmid

#248787

Purpose

fuse partial UTR and coding sequence of Brd9 (zebrafish) with EGFP reporter

Depositor

Wenyan Ren

Article

Wu et al Commun Biol. 2025 Dec 1. doi: 10.1038/s4

Insert

brd9 (brd9 Zebrafish)

Use

Brd9(zebrafish)-egfp reporter

Tags

EGFP

Promoter

partial 5'UTR sequence of zebrafish brd9

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-cOpn5-T2A-EGFP

Plasmid

#237858

Purpose

The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV and Cre/Lox

Promoter

EF1α

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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