We narrowed to 14,247 results for: RON

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pCGN-ELK-1 Ets mut

Plasmid

#27157

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

R62E/R65E/Y66D

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP

Plasmid

#21849

Depositor

David Ron

Insert

ATF4 (Atf4 Mouse)

Tags

myc

Expression

Mammalian

Mutation

N-terminal transactivation domain deleted and rep…

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-21-5p

Plasmid

#103345

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-21-5p target (MIR21 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human full-length LIC1

Plasmid

#74597

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCGN-S2P-WT

Plasmid

#32957

Purpose

For mammalian expression of human S2P with an HA tag

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

S2P (MBTPS2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only
pPSSUB-101

Plasmid

#26580

Depositor

Ron Weiss

Article

Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 1

Insert

destabilized GFP

Expression

Bacterial

Mutation

S2R, C-terminal extension RPAANDENYA(LVA)

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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DNA-CARzeta-IRESpuro

Plasmid

#89345

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta

Use

Lentiviral

Tags

SNAPf tag

Expression

Mammalian

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTK296

Plasmid

#59565

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP-PEST

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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NCD-GFP

Plasmid

#24507

Depositor

Ron Vale

Article

Case et al Cell. 1997 Sep 5. 90(5):959-66.

Insert

NCD (ncd Fly)

Tags

GFP and His

Expression

Bacterial

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
IRE1b(K536A).9E10.pCDNA3

Plasmid

#21881

Depositor

David Ron

Insert

IRE1b (Ern2 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

K536A

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only

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DNA-CARtcr-IRESpuro

Plasmid

#89346

Purpose

2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag

Use

Lentiviral

Tags

SNAPf tag

Expression

Mammalian

Promoter

SFFV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-Kif18b-24xGCN4_v1

Plasmid

#72228

Purpose

A fusion of the Kif18b motor protein to the v1 SunTag peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Kif18b (KIF18B Human)

Expression

Mammalian

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pTK332

Plasmid

#59573

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTK295

Plasmid

#59564

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRFP

Plasmid

#26924

Depositor

Ron Weiss

Article

Basu et al Nature. 2005 Apr 28. 434(7037):1130-4.

Insert

LacI, HcRed

Expression

Bacterial

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pET_Unc653_GFP_His

Plasmid

#14107

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (1-653) (unc-104 Nematode)

Tags

GFP and His

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTK194

Plasmid

#59563

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak mKate opal attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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