We narrowed to 2,459 results for: GEM

1. pGFP-Ub-VPS13D_dC

   Plasmid

   #176466

   Purpose

   express VPS13D lacking C-terminal 407 aa in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Use

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   C-terminal 407 aa is deleted.

   Promoter

   CMV

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESUMO-hRORg2

   Plasmid

   #170329

   Purpose

   Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

   Depositor

   Patrick Griffin

   Article

   Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

   Insert

   RORG isoform 2 (RORC Human)
   

   Use

   Tags

   HisSUMO

   Expression

   Bacterial

   Mutation

   codon optimized

   Promoter

   T7

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBL3664

   Plasmid

   #162240

   Purpose

   Transcribes E. coli SRP RNA sequence for SHAPE-Seq

   Depositor

   Julius Lucks

   Article

   Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

   Inserts

   E. coli SRP RNA
   HDV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-Akt2(K14A)

   Plasmid

   #86624

   Purpose

   Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2(K14A) (AKT2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-NDUFB4

   Plasmid

   #139840

   Purpose

   Lentiviral expression of human NDUFB4

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   NDUFB4 (NDUFB4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

   Plasmid

   #52878

   Purpose

   Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E90R, D156N, T159C

   Promoter

   CaMKIIa

   Available since

   July 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

   Plasmid

   #238372

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cmlc2 (myl7)

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol1-Spe-KpnI-ubb-nmKate

   Plasmid

   #238373

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYCDuet-1:ChrA-C63S-C66S

   Plasmid

   #225082

   Purpose

   Expresses precursor peptide ChrA-C63S/C66S in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA-C63S/C66S
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C63S/C66S

   Promoter

   T7

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYCDuet-1:ChrA-C66S

    Plasmid

    #225081

    Purpose

    Expresses precursor peptide ChrA-C66S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C66S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C66S

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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