Addgene

We narrowed to 22,017 results for: his

Showing: 3391 - 3420 of 22017 results

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pAc5.1B-lambdaN-HA-DmSmaug_584-999-V5His6_C

Plasmid

#146012

Purpose

Insect Expression of DmSmaug_584-999

Depositor

Elisa Izaurralde

Insert

DmSmaug_584-999 (smg Fly)

Use

Tags

Expression

Insect

Mutation

5 silent mutations and a deletion of the AA 961-9…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSmaug-A642H-V5His6_C

Plasmid

#146014

Purpose

Insect Expression of DmSmaug-A642H

Depositor

Elisa Izaurralde

Insert

DmSmaug-A642H (smg Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSmaug-K606AR609A-V5His6_C

Plasmid

#146016

Purpose

Insect Expression of DmSmaug-K606AR609A

Depositor

Elisa Izaurralde

Insert

DmSmaug-K606AR609A (smg Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-gamma-tubulin-TEV-His6

Plasmid

#178067

Purpose

Insect cell expression of gamma-tubulin-TEV-His6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin (TUBG1 Human)

Use

Tags

TEV-His6

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSumo-His6- Sumo-OsTPL(1-209)

Plasmid

#177855

Purpose

Bacterial Expression of TPL

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPL

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/6xHis mCherry F30 Broccoli

Plasmid

#169234

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-Broccoli

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-NTerminus8xHis-T500

Plasmid

#178421

Purpose

Empty backbone. T7Max promoter with N-His Tag

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-AtTPR2(1-209)

Plasmid

#177860

Purpose

Bacterial Expression of TPR2

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR2 (TPR2 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-OsTPR2(1-210)

Plasmid

#177859

Purpose

Bacterial Expression of TPR2

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR2

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.3 (19-361)

Plasmid

#177846

Purpose

Bacterial Expression of SnRK2.3

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET Duet1 His6 SUMO D14

Plasmid

#177854

Purpose

Bacterial Expression of D14

Depositor

Karsten Melcher

Article

Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

Insert

D14

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET24a His6 GST PYL2(14-188)

Plasmid

#177842

Purpose

Bacterial Expression of PYL2

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 2 (PYL2 Mustard Weed)

Use

Tags

6xHis-GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-DSep1-msfGFP

Plasmid

#174494

Purpose

bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

Sep1 (Sep1 Fly)

Use

Tags

His6-TEV and msfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgβ-anticalin-His-ybbr

Plasmid

#168040

Purpose

Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Use

Tags

6x Histag, Fgβ peptide, and ybbr tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Use

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

Plasmid

#146166

Purpose

Insect Expression of DmUPF2-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF2-siRNAres (Upf2 Fly)

Use

Tags

Expression

Insect

Mutation

3 non silent mutations: L1022P, V1097L and N1213S…

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

Plasmid

#146013

Purpose

Insect Expression of DmSmaug_763-999

Depositor

Elisa Izaurralde

Insert

DmSmaug_763-999 (smg Fly)

Use

Tags

Expression

Insect

Mutation

2 silent mutations and a deletion of the AA 961-9…

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

Plasmid

#146001

Purpose

Insect Expression of DmSmaug_1-762

Depositor

Elisa Izaurralde

Insert

DmSmaug_1-762 (smg Fly)

Use

Tags

V5-6xHis

Expression

Insect

Mutation

NP_523987.1, 7 silent mutations compared to the s…

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_dead_mt

Plasmid

#167270

Purpose

Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

C145A

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast_dead_mt

Plasmid

#167272

Purpose

Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

E30R-C145A

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQC hKGA.S286A V5-His IRES G418

Plasmid

#110391

Purpose

γ-Retroviral transfer vector for expressing glutminase isoform KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

S286A

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R459E

Plasmid

#164024

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R459E

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R464E

Plasmid

#164025

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R464E

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E485R

Plasmid

#164026

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E485R

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E490R

Plasmid

#164027

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E490R

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R495E

Plasmid

#164028

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R495E

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C D511K

Plasmid

#164029

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, D511K

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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