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Showing: 5651 - 5700 of 167150 results

  1. Emerald-Lysosomes-20

    Plasmid
    #56476
    Purpose
    Localization: Lysosome Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Tags
    Emerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. Vinculin-23

    Plasmid
    #58198
    Purpose
    Localization: Focal Adhesions, Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Tags
    SNAP tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. tdTurboRFP-Lysosomes-20

    Plasmid
    #58061
    Purpose
    Localization: Lysosome Membrane, Excitation: 553, Emission: 576
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Tags
    tdTurboRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    58061-plasmid-map-sequence-id-260713

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  4. EGFP-hCALML3

    Plasmid
    #47604
    Depositor
    Emanuel Strehler
    Insert
    CALML3 (CALML3 Human)
    Tags
    EGFP-HA-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pSOX2.1.0-gDNA

    Plasmid
    #176382
    Purpose
    Expresses spCas9 and gRNA sequence targeting SOX2
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SOX2 gRNA
    Use
    CRISPR
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176382-plasmid-map-sequence-id-347388

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  6. pPs-neo HA-RalA

    Plasmid
    #20181
    Depositor
    William Hahn
    Insert
    RalA (RALA Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G23V (constitutively active), E147D
    Available Since
    March 3, 2009
    Availability
    Academic Institutions and Nonprofits only
    20181-plasmid-map-sequence-id-207517

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  7. EGFP-LC-Myosin-N-7

    Plasmid
    #56282
    Purpose
    Localization: Light Chain Myosin, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    LC-Myosin
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pTU2-a_SKA_amilCP

    Plasmid
    #204055
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101 (WT) ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204055-plasmid-map-sequence-id-413881

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  9. mRuby-LC-Myosin-C-10

    Plasmid
    #55870
    Purpose
    Localization: Light Chain Myosin, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    LC-Myosin
    Tags
    mRuby
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55870-plasmid-map-sequence-id-266826

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  10. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Available Since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

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  11. mApple-Talin-C-18

    Plasmid
    #62738
    Purpose
    Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:568, Emission:592
    Depositor
    Michael Davidson
    Insert
    Talin
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. ETV1A-c014

    Plasmid
    #53655
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    ETV1A
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. PA-sfGFP-C1

    Plasmid
    #54578
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 485, Emission: 468 / 511
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-sfGFP
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. T7RNAP Cyslight A188C

    Plasmid
    #205083
    Purpose
    T7RNAP protein expression
    Depositor
    Smita Patel
    Insert
    T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
    Expression
    Bacterial
    Mutation
    C125S, C347S, C510S, C515S, C530S, C723S N88C
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205083-plasmid-map-sequence-id-413708

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  15. sfGFP-FAK-5

    Plasmid
    #56484
    Purpose
    Localization: Focal Adhesion Kinase, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    FAK
    Tags
    sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56484-plasmid-map-sequence-id-226423

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  16. pEF1-His-NEDD8

    Plasmid
    #159322
    Purpose
    Mammalian cell expression of mouse His-NEDD8
    Depositor
    Jianjun Zhao
    Insert
    His-NEDD8 (Nedd8 Mouse)
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159322-plasmid-map-sequence-id-311864

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  17. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  18. mStrawberry-Cx43-7

    Plasmid
    #55937
    Purpose
    Localization: Gap Junctions, Excitation: 574, Emission: 596
    Depositor
    Michael Davidson
    Insert
    Cx43
    Tags
    mStrawberry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55937-plasmid-map-sequence-id-446220

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  19. pSP019

    Plasmid
    #168195
    Purpose
    expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.
    Depositor
    James Fleckenstein
    Insert
    eatA[H134R]
    Expression
    Bacterial
    Mutation
    H134R mutation
    Promoter
    araBAD
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    168195-plasmid-map-sequence-id-339468

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  20. lenti.Cas9.BFP.sgRNA.parental

    Plasmid
    #196715
    Purpose
    Sortable WT-SpCas9 expression and guide cassette (1-vector system).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196715-plasmid-map-sequence-id-389692

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  21. pETM41-EcPPK

    Plasmid
    #38334
    Depositor
    Florian Freimoser
    Insert
    E. coli polyphosphate kinase (ppk Escherichia coli)
    Tags
    6xHIS, MBP, and TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  22. mEos3.2-MyosinIIA-C-18

    Plasmid
    #57475
    Purpose
    Localization: Cytoskeleton, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MyosinIIA
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. pDEST40-DJ1-C106A

    Plasmid
    #29409
    Depositor
    Mark Cookson
    Insert
    DJ1 (PARK7 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Available Since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only

  24. mEmerald-Plastin-C-10

    Plasmid
    #54232
    Purpose
    Localization: Fimbrin/Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Plastin (PLS1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pSB-Bleo STAT3

    Plasmid
    #139184
    Purpose
    Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    STAT3
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    139184-plasmid-map-sequence-id-309370

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  26. Nup54_mEGFP494(-4)

    Plasmid
    #163432
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 4 amino acids) at amino acid position 494
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163432-plasmid-map-sequence-id-321674

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  27. mVenus-Lifeact-7

    Plasmid
    #54688
    Purpose
    Localization: Actin, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Lifeact-mVenus
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pKM263-EcPPK

    Plasmid
    #38333
    Depositor
    Florian Freimoser
    Insert
    E. coli polyphosphate kinase (ppk Escherichia coli)
    Tags
    6xHIS, GST, and TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    38333-plasmid-map-sequence-id-48396

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  29. pBSVSG221KOblast

    Plasmid
    #59733
    Purpose
    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.
    Depositor
    Gloria Rudenko
    Insert
    VSG221 knockout construct
    Use
    For homologous recombination into vsg221 expressi…
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    59733-plasmid-map-sequence-id-93744

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  30. ADH1pro_GCN4_SIR4

    Plasmid
    #138620
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCN4 (GCN4 Budding Yeast)
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    138620-plasmid-map-sequence-id-334951

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  31. mEmerald-LASP2-C-18

    Plasmid
    #54143
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    LASP2 (NEBL Chicken)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. pDONR2T18ms

    Plasmid
    #72645
    Purpose
    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Available Since
    Feb. 29, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72645-plasmid-map-sequence-id-133017

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  33. Dendra1-N1

    Plasmid
    #54624
    Purpose
    Localization: N1 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Dendra1
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. R777-E263 Hs.RASSF6

    Plasmid
    #70547
    Purpose
    Gateway ORF clone of human RASSF6 [NM_177532.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF6 (RASSF6 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70547-plasmid-map-sequence-id-125996

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  35. mCherry-AuroraA-C-7

    Plasmid
    #54997
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    AuroraA
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I57V in AuroraA
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. sfGFP-C-Src-7

    Plasmid
    #56478
    Purpose
    Localization: Membrane/Vesicles, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    C-Src
    Tags
    sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid
    #92296
    Purpose
    Expression of humanized FbCpf1
    Depositor
    Feng Zhang
    Insert
    huFbCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92296-plasmid-map-sequence-id-181502

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  38. Peroxisomes-FAST

    Plasmid
    #216343
    Purpose
    For testing localization of pFAST protein to peroxisomes
    Depositor
    Alison Tebo
    Insert
    pFAST_SKL
    Tags
    myc for FAST protein
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available Since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216343-plasmid-map-sequence-id-427401

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  39. CUL3A-c012

    Plasmid
    #53673
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    CUL3A
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. mApple-FAK-5

    Plasmid
    #54898
    Purpose
    Localization: Focal adhesion kinase, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    FAK
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. pShuttle-Flag-HA

    Plasmid
    #63681
    Purpose
    adenoviral shuttle vector containing N-terminal Flag tag
    Depositor
    Qingbo Xu, Lingfang Zeng
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    63681-plasmid-map-sequence-id-263358

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  44. EYFP-Lysosomes-20

    Plasmid
    #56595
    Purpose
    Localization: Lysosome Membrane, Excitation: 513, Emission: 527
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. pmCer-TopBP1

    Plasmid
    #211412
    Purpose
    mammalian expression of human TopBP1 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211412-plasmid-map-sequence-id-420515

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  46. mEmerald-Plastin-N-10

    Plasmid
    #54233
    Purpose
    Localization: Fimbrin/Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Plastin (PLS1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. L3691

    Plasmid
    #1587
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    mec7-836, mec7Tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1587-plasmid-map-sequence-id-197

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  48. Dendra1-C1

    Plasmid
    #54689
    Purpose
    Localization: C1 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Dendra1
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. SEMA3E (mouse) pBLUE

    Plasmid
    #29453
    Depositor
    Jonathan Raper
    Insert
    Sema3E (mouse) (Sema3e Mouse)
    Use
    Pblue for ish
    Mutation
    1400bp fragment for ISH
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  50. FUW-mTie1

    Plasmid
    #119234
    Purpose
    Plasmid encodes full-length murine Tie1 gene cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    Tie1 (Tie1 Mouse)
    Use
    Lentiviral
    Promoter
    P hUbC
    Available Since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    119234-plasmid-map-sequence-id-225493

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Showing: 5651 - 5700 of 167150 results