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pENTR-D-Topo-kcnk12

Plasmid

#208995

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk12 (kcnk12 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS

Plasmid

#115250

Purpose

For in vitro transcription of human NODAL open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTa-HH7-2

Plasmid

#124997

Purpose

To make transgenic mice for HH7-2 TCRa

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-2 TCRa Tg

Use

Mouse Targeting

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTa-HH5-1

Plasmid

#124995

Purpose

To make transgenic mice for HH5-1 TCRa

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1 TCRa Tg

Use

Mouse Targeting

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNfs1

Plasmid

#102967

Purpose

Suppression of mNfs1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNfs1

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pWJPrP34

Plasmid

#22107

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP exon 3 EcoR1 fragment

Use

Construction of targeting vector

Mutation

Added a Cla1 site right before translation start …

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFuse-STOP

Plasmid

#217677

Purpose

Empty vector control plasmid containing a stop codon prior to the Fc encoding region

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Type

Empty backbone

Expression

Mammalian

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427V

Plasmid

#81044

Purpose

mammalian expression of LOV2 I427V

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427V

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427V

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416T

Plasmid

#81040

Purpose

mammalian expression of LOV V416T

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2 V416T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag9

Plasmid

#175543

Purpose

Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast

Plasmid

#109334

Purpose

pInducer20 with added blasticidin resistance

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Type

Empty backbone

Use

Lentiviral; Inducible expression

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_HaloTag9

Plasmid

#175545

Purpose

Expression of HaloTag9 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

Plasmid

#175544

Purpose

Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag9-myc_PDGFRtmb

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_SKL

Plasmid

#175542

Purpose

Peroxysomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-SKL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

Plasmid

#175540

Purpose

Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag9-KDEL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_HaloTag9

Plasmid

#175541

Purpose

Lyzosomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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