We narrowed to 44,084 results for: cha

1. pOPINK-OTUD6B (OTU, aa 133-293)

   Plasmid

   #61419

   Purpose

   Expresses human OTUD6B (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6B (OTUD6B Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-132.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-HOXA9

   Plasmid

   #131608

   Purpose

   expression of human HOXA9 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   HOXA9 (HOXA9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXs_FLAG-FSF1

   Plasmid

   #110641

   Purpose

   Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   FSF1 (FSF1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Promoter

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mEos2-Talin-IBS2-7

   Plasmid

   #57424

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Talin IBS2 (Tln1 Mouse)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Talin Integrin Binding Site

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pFA6a-kanMX6-P41nmt1-GST

   Plasmid

   #39287

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pOPINK-OTUD2 (OTU, aa 132-314)

   Plasmid

   #61410

   Purpose

   Expresses human OTUD2 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD2 (YOD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-131 and aa 315-348.

   Promoter

   T7

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. UASgal-A9-Pleum

   Plasmid

   #60343

   Purpose

   Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCfB3053(gRNA X-2, XI-5, XII-4)

   Plasmid

   #73295

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS human Frataxin (myc tag)

   Plasmid

   #11443

   Depositor

   Ronald Kahn

   Article

   Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

   Insert

   Frataxin (FXN Human)
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pMXs_FLAG-ODC2

    Plasmid

    #133257

    Purpose

    Express FLAG-tagged ODC2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC2 (ODC2 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXs_FLAG-NDT2

    Plasmid

    #133255

    Purpose

    Express FLAG-tagged NDT2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT2 (YEA6 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBabe puro human Frataxin

    Plasmid

    #11505

    Depositor

    Ronald Kahn

    Article

    Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Frataxin (FXN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pHL 1917

    Plasmid

    #53024

    Purpose

    AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    mCherry
    fimB (fimB E. coli)
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, RBS(st7), and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pCon and pLlacO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. QuasAr-P2A-GCaMP

    Plasmid

    #154082

    Purpose

    Independent expression of QuasAr and GCaMP under human synapsin 1 promoter

    Depositor

    Michael Hoppa

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

    Insert

    QuasAr-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 327 to Alanine

    Promoter

    human synapsin 1

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS316-VTC4

    Plasmid

    #108691

    Purpose

    VTC4 sequence expressed in yeast plasmid

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Vacuolar Transporter Chaperone 4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    VTC4 (native promoter)

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid

    #128256

    Purpose

    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Mouse c-myc (exon I)

    Plasmid

    #8446

    Depositor

    Frederic Mushinski

    Article

    Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

    Insert

    Mouse c-myc (exon I) (Myc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

18. BRCA1-(1234-1443)

    Plasmid

    #35078

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains aa 1234-1443

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

19. r-22. pT7-7-SNCA-9Asn

    Plasmid

    #107297

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-9Asn (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. t2. pT7-7-SNCA-5Lys

    Plasmid

    #107294

    Purpose

    Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Lys (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. r-6. pT7-7-SNCA-2Asn

    Plasmid

    #107296

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-2Asn (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. r. pT7-7-SNCA-5Asn

    Plasmid

    #107295

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Asn (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. s2. pT7-7-SNCA-5Asp

    Plasmid

    #107293

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Asp (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-BE3-2X

    Plasmid

    #110835

    Purpose

    CMV expression vector for BE3-2X construct (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-2X
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2 tandem NLS sequences in the XTEN linker

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWHI2-NAT

    Plasmid

    #80588

    Purpose

    CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

    Depositor

    John McCusker

    Article

    Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

    Insert

    WHI2 (WHI2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Native WHI2 promoter

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pUDP010

    Plasmid

    #101166

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGS1212

    Plasmid

    #63785

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Strep-tag II followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEYFP-N1-hRIP3-FL VV458/460PP

    Plasmid

    #61368

    Purpose

    Expresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with VV458/460P mutation (RIPK3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V458 to P and V460to P

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pHcRed-miR-125b-1

    Plasmid

    #58989

    Purpose

    MSCV based expression vector - co-expresses HcRed and human miR-125b-1

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Inserts

    HcRed fluorescent protein
    miR-125b-1 (MIR125B1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pEGFP-N1-hRIP1-FL I539P

    Plasmid

    #61380

    Purpose

    Expresses Human full length RIPK1with I539P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP1with I539P mutation (RIPK1 Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    changed I539 to P

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

31. BRCA1-(1057-1234)

    Plasmid

    #35076

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains aa 1057-1234

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pBabe hygro human Frataxin

    Plasmid

    #11504

    Depositor

    Ronald Kahn

    Article

    Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Frataxin (FXN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pBabe neo human Frataxin

    Plasmid

    #11503

    Depositor

    Ronald Kahn

    Article

    Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Frataxin (FXN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pYO843

    Plasmid

    #242487

    Purpose

    Expression of ScAtg38 Δ(210 to 226)-2xEGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    ATG38 (YLR211C Budding Yeast)
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    deletion of aa 210 to 226

    Promoter

    Available Since

    Sept. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYO835

    Plasmid

    #242484

    Purpose

    Expression of ScAtg38 Δ(123 to 181)-2xEGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    ATG38 (YLR211C Budding Yeast)
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    deletion of aa 123 to 181

    Promoter

    Available Since

    Sept. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYO842

    Plasmid

    #242486

    Purpose

    Expression of ScAtg38 Δ(123 to 209)-2xEGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    ATG38 (YLR211C Budding Yeast)
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    deletion of aa 123 to 209

    Promoter

    Available Since

    Sept. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYO817

    Plasmid

    #242483

    Purpose

    Expression of ScAtg38 Δ(182 to 226)-2xEGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    ATG38 (YLR211C Budding Yeast)
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    deletion of aa 123 to 226

    Promoter

    Available Since

    Sept. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYO836

    Plasmid

    #242485

    Purpose

    Expression of ScAtg38 Δ(182 to 209)-2xEGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    ATG38 (YLR211C Budding Yeast)
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    deletion of aa 182 to 209

    Promoter

    Available Since

    Sept. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-cNM2A-Flag

    Plasmid

    #236532

    Purpose

    For expression of NM2A (dog) - with HA and FLAG tags in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    MYH9
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HA-mNM2A

    Plasmid

    #236531

    Purpose

    For expression of NM2A (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    MYH9 (Myh9 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRDA_052_HygroR

    Plasmid

    #228937

    Purpose

    Plasmid for cloning of custom gRNA using EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCRISPEY-Z3-Nat-ADE2

    Plasmid

    #232106

    Purpose

    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    Z3

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCRISPEY-GAL-Hyg-ADE2

    Plasmid

    #232104

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCRISPEY-Z3-Hyg-ADE2

    Plasmid

    #232107

    Purpose

    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    Z3

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLentiCRISPR-v1-sgMTHFD1

    Plasmid

    #217436

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting MTHFD1 (MTHFD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLJC2-TK2-3xFLAG

    Plasmid

    #217427

    Purpose

    Lentiviral overexpression of human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    TK2 (TK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Insert sequence is a codon-optimized gBLOCK (IDT)

    Promoter

    CMV

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLentiCRISPR-v1-sgTYMS

    Plasmid

    #217430

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting TYMS (TYMS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLentiCRISPR-v1-sgTK2_4

    Plasmid

    #217431

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 4 targeting TK2 (TK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLentiCRISPR-v1-sgTK2_5

    Plasmid

    #217432

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 5 targeting TK2 (TK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLentiCRISPR-v1-sgSHMT2

    Plasmid

    #217435

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting SHMT2 (SHMT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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