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Plasmid#68180PurposeProvides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid partDepositorInsert4xOpLexA:mini35S
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTHsp18.2 (GB0035)
Plasmid#68186PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-pET-RBS
Plasmid#72981PurposePET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)DepositorInsertpET RBS derived from pET vectors
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI Methylase-AmilCP
Plasmid#85166PurposeFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.DepositorInsertEcoRI Methylase-AmilCP
UseTagsAmilCPExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpLacI:mini35ss (GB0534)
Plasmid#68179PurposeProvides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid partDepositorInsert6xOpLacI:mini35S
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pISH-Dlx3
Plasmid#74342PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.DepositorInsertdistal-less homeobox 3 (Dlx3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUPD_OsU3 (GB1184)
Plasmid#68209PurposeProvides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertOsU3 promoter
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Meis2
Plasmid#74349PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Meis2. Not for gene expression.DepositorInsertMeis homeobox 2 (Meis2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHcPro (GB0067)
Plasmid#68196PurposeProvides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid partDepositorInsertTEV HcPro coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pISH-Cux1
Plasmid#74341PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.DepositorInsertcut-like homeobox 1 (Cux1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Gucy1b3
Plasmid#105458Purposeto generate a riboprobe for in situ hybridazationDepositorInsertGucy1b3 (Gucy1b1 Mouse)
UseTo ganerate riboprobeTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISH-Meis1
Plasmid#74348PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Meis1. Not for gene expression.DepositorInsertMeis homeobox 1 (Meis1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFL-TK-AID3'UTR-MUT
Plasmid#19987DepositorInsertAID 3' UTR (Aicda Mouse)
UseTagsExpressionMammalianMutationMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only