We narrowed to 5,473 results for: KRAS

1. p2T-cmv-SpCas9-TadDE-BlastR

   Plasmid

   #193850

   Purpose

   Express TadDE in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

   Plasmid

   #222917

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA179

   Plasmid

   #215939

   Purpose

   Fragmid fragment: (guide cassette) short sequence with no known function

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Filler
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP-pk-S65A

   Plasmid

   #115619

   Purpose

   Expresses human Parkin S65A in mammalian cells

   Depositor

   Edward Fon

   Article

   Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

   Insert

   Parkin (PRKN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S65A

   Promoter

   CMV

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA331

   Plasmid

   #215954

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT9752-BEAR-mScarlet

   Plasmid

   #162991

   Purpose

   BEAR target plasmid with split mScarlet and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mScarlet split with an intron between amino acids 110-111
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-gRNA(anti-cGAS)-Cas9-Puro

   Plasmid

   #130908

   Purpose

   gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2CG2-QUASM1:GFPNLS-SV40pA

   Plasmid

   #155123

   Purpose

   Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1:GFPNLS
   

   Use

   Zebrafish expression

   Promoter

   QUASM1-E1b

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_831

   Plasmid

   #216100

   Purpose

   CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pProEx Rheb(R15C) CL

    Plasmid

    #224266

    Purpose

    Protein purification of Rheb (R15C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rheb (RHEB Human)
    

    Expression

    Bacterial

    Mutation

    R15C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-TKFC in pLJM60

    Plasmid

    #154206

    Purpose

    Lentiviral vector for stable expression of TKFC

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    TKFC (DAK Human)
    

    Use

    Lentiviral

    Tags

    N terminal FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-Caveolin-N-10

    Plasmid

    #54026

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

    Insert

    Caveolin (CAV1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pcDNA5FRT/TO-UBXD7-Strep-HA

    Plasmid

    #113479

    Purpose

    Expression of human UBXD7 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD7 (UBXN7 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    A834C silent mutation, wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA008

    Plasmid

    #216003

    Purpose

    Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas12a_v1.1 [EnAs]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178574

    Purpose

    Lentiviral vector for CamKIIα::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    Lentiviral

    Promoter

    CaMKIIa

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CAG-KKHSaCas9-BlastR

    Plasmid

    #107189

    Purpose

    Confers constitutive expression of KKH SaCas9.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    KKH SaCas9 (NEWENTRY )
    

    Expression

    Bacterial

    Mutation

    E782K/N968K/R1015H

    Promoter

    Chicken ß-Actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRT/TO-UBE2QL1-Strep/HA

    Plasmid

    #124666

    Purpose

    Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

    Plasmid

    #130708

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
    

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5FRT/TO-p37-Strep-HA

    Plasmid

    #113485

    Purpose

    Expression of human p37 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p37 (UBXN2B Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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