We narrowed to 7,126 results for: ef1a

1. AsdCas12a VPR

   Plasmid

   #188512

   Purpose

   Expresses FLAG tagged AsdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL057

   Plasmid

   #177169

   Purpose

   Expresses a nuclease dead MRE11 in human cells

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   MRE11 H129N (MRE11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   nuclease-inactivating mutation (H129N)

   Promoter

   EF1a

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS-HsProm1s1-StayGold

   Plasmid

   #211333

   Purpose

   Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   AAV

   Tags

   StayGold

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-RT006-GCN4-HaloPM

   Plasmid

   #163386

   Purpose

   To express 4X sGCN4 module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-IGK-dAPEX2-KDEL

   Plasmid

   #117182

   Purpose

   Plasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   IGK-dAPEX2-KDEL
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBbi_RP_MicroID2-FLAG-NES

   Plasmid

   #188525

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRai

   Plasmid

   #189938

   Purpose

   Expresses CRISPRai cassette under EF1a promoter

   Depositor

   Yu-Chen Hu

   Article

   Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

   Insert

   CRISPRai cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer-rat EF1 alpha

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSimpleII-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47867

   Purpose

   This plasmid encode NmCas9 with two NLS and a HA tag

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR RPS27A-3xFlag_IRES-GFP

   Plasmid

   #198390

   Purpose

   RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FDIO-COX4-dAPEX2

    Plasmid

    #117178

    Purpose

    Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    COX4-dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-NES-mTagBFP2

    Plasmid

    #216129

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-MAPKKnucelarExportSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HiFi dCas9 KRAB

    Plasmid

    #188500

    Purpose

    Expresses FLAG tagged HiFi dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP in pDBEcR-pIND(SP1)/Bla

    Plasmid

    #201557

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc and FLAG-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pIMD(SP1)

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FDIO-LACTB-dAPEX2

    Plasmid

    #117181

    Purpose

    Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    LACTB-dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX311 LacZ

    Plasmid

    #117731

    Purpose

    Open reading frame vector encoding LacZ

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2X V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PlmdCas12e KRAB

    Plasmid

    #188506

    Purpose

    Expresses FLAG tagged PlmdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-KRAB-NLS

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

    Plasmid

    #114376

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP, Kv2.1C, and TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBB-BT-Ubiquitin

    Plasmid

    #36098

    Depositor

    Ezra Burstein

    Article

    Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

    Insert

    Ubiquitin (RPS27A Human)
    

    Use

    Tags

    TB-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FDIO-SYP-HRP

    Plasmid

    #117187

    Purpose

    Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-eDIO-ChR2-EYFP

    Plasmid

    #104387

    Purpose

    PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

    Depositor

    Volker Busskamp

    Article

    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

    Insert

    Channelrhodopsin 2
    

    Use

    Cre/Lox; Piggybac

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E134R

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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