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mCherry-INPP5B

Plasmid

#171947

Purpose

Expresses INPP5B in mammalian cells

Depositor

Cheng-han Yu

Publication

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5B (INPP5B Human)

Tags

mCherry

Expression

Mammalian

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFnCpf1GG

Plasmid

#131010

Purpose

Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Publication

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of FnCas12a
GFP expression cassette

Use

CRISPR

Expression

Bacterial

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG010

Plasmid

#113984

Purpose

Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

RK2 (IncP)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7T-EcadTSMod

Plasmid

#99871

Purpose

RNA synthesis for microinjection into xenopus laevis embryo

Depositor

Marc Tramier

Publication

Herbomel et al Sci Rep. 2017 Mar 22;7:45058. doi: 10.10

Insert

EcadTSMod

Use

Xenopus microinjection

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCherry-EF(C)

Plasmid

#61664

Purpose

Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)

Expression

Mammalian

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pPGK-GZF1-RR

Plasmid

#35092

Depositor

David Segal

Publication

Bhakta et al Methods Mol Biol. 2010;649:3-30.

Insert

ZFN GZF1-Fok(RR)

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

contains the "RR" obligate heterodimer …

Promoter

PGK

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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nGCaMP2-NpuDnaEn

Plasmid

#34850

Depositor

Kevin Truong

Publication

Wong et al Cell Calcium. 2011 Nov 29.

Insert

nGCaMP2-NpuDnaEn

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
HS-H4-GFP

Plasmid

#8699

Depositor

Kami Ahmad

Publication

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H4 (His4:CG31611 Fly)

Tags

GFP

Expression

Insect

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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mEGFP-PLIN3

Plasmid

#222426

Purpose

To express PLIN3 with mEGFP tag in mammalian cells

Depositor

Stephen Royle

Publication

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

PLIN3 (PLIN3 Human)

Tags

mEGFP

Expression

Mammalian

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-FRISZ

Plasmid

#176887

Purpose

Far-red fluorescent zinc indicator FRISZ in pDisplay

Depositor

Huiwang Ai

Publication

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV HA-DP-1

Plasmid

#133705

Purpose

Expression of HA-tagged TFDP1.

Depositor

James A. DeCaprio

Publication

Krek et al Science. 1993 Dec 3. 262(5139):1557-60.

Insert

TFDP1 (TFDP1 Human)

Tags

HA

Expression

Mammalian

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-msCFPx6

Plasmid

#105268

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

msCFPx6

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TAL-L1-2.4-Ctrl

Plasmid

#103037

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

Depositor

Maria-Elena Torres-Padilla

Publication

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-2.4

Use

T3 in vitro transcription for expression in mamma…

Tags

FLAG and HA

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Reb_221 aka PAS628

Plasmid

#71233

Purpose

Reb_1 with RebA(G90A)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, G90A)

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pMR150

Plasmid

#32292

Depositor

Verne Chapman, Rudolf Jaenisch

Publication

Sanford et al Genes Dev. 1987 Dec;1(10):1039-46.

Insert

genomic methylation probe-minor satellite repeat

Expression

Bacterial

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pKH3-avian RSK1

Plasmid

#8998

Depositor

John Blenis

Publication

Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

Insert

RSK (RPS6KA1 Chicken)

Tags

3x HA

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mCherry-APPP1-mGFP

Plasmid

#196705

Purpose

As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

Depositor

Martino Calamai

Publication

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

mCherry and mEGFP

Expression

Mammalian

Mutation

Lys to Val substitution at position 612

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Publication

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Expression

Mammalian

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas9+sgRNA expression vector precursor

Plasmid

#171798

Purpose

PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

Depositor

Stephen Elledge

Publication

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO

Available since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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