We narrowed to 14,886 results for: pET

1. pet-ECHB(35-475)

   Plasmid

   #96890

   Purpose

   expresses His-tagged mouse ECHB (35-475) in E.coli cells

   Depositor

   Hening Lin

   Article

   Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

   Insert

   Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-34

   Promoter

   T7

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET20b-delta133HtrA2 S142D

   Plasmid

   #16154

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S142D

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pET3a pro-hRegIIIa

   Plasmid

   #64935

   Purpose

   bacterial expression of full length human RegIIIalpha

   Depositor

   Lora Hooper

   Article

   Cash et al Protein Expr Purif. 2006 Jul;48(1):151-9

   Insert

   RegIIIa (REG3A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations made in 5' end to relax secondary …

   Promoter

   T7

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET28-his-3C_Clu3B6p

   Plasmid

   #100093

   Purpose

   expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

   Depositor

   Nikolai Sluchanko

   Article

   Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

   Insert

   14-3-3 sigma human (SFN Human)
   

   Use

   Tags

   HSPB6 peptide and His6

   Expression

   Bacterial

   Mutation

   75-77 residues changed to AAA to facilitate cryst…

   Promoter

   T7

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET20b- hTPI Ile170Val

   Plasmid

   #50724

   Purpose

   overexpression of the human TPI1 Ile170Val allele in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   changed Isoleucine 170 to Valine

   Promoter

   T7

   Available since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. HOXA4 (murine) pET

   Plasmid

   #8488

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXA4 (Hoxa4 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Thr-2 changed to Ala-2 to form better ribosomal b…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pET11d-FHis-DND1

   Plasmid

   #70083

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Use

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6his R.3-AR GUSpET28a+/pLysS

   Plasmid

   #61157

   Purpose

   N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

   Depositor

   Andrew Ellington, Ichiro Matsumura

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   GUSAR
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

   Promoter

   T7

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET20b-CeNLuc-6xHis

   Plasmid

   #135933

   Purpose

   Expresses the CeNLuc LumiFluor in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   CeNLuc LumiFluor
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET20b-delta133HtrA2 S400A

    Plasmid

    #16155

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S400A

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pET21-Drp1(deltaIB)

    Plasmid

    #73041

    Purpose

    Bacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Drp1(deltaIB) (Dnm1l Mouse)
    

    Use

    Tags

    6xHis-tag and PreScission Protease site

    Expression

    Bacterial

    Mutation

    Deleted amino acids 514-602

    Promoter

    T7

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET11a+D77R/T214V

    Plasmid

    #66135

    Purpose

    Expresses the D77R/T214V mutant of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HM3C NaVSp1_p pET24b

    Plasmid

    #127852

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    3C Cleavage site, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-FLAG-eSpCas9

    Plasmid

    #126769

    Purpose

    Expression of increased fidelity eSpCas9 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet-msGC-NT13

    Plasmid

    #75087

    Purpose

    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

    Depositor

    William Montfort

    Article

    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

    Inserts

    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    fragment 1-380 of the beta subunit and fragment 4…

    Promoter

    Available since

    May 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-Hook3 aa 1-160

    Plasmid

    #74607

    Purpose

    Expresses Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFS_0385_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1

    Plasmid

    #116997

    Purpose

    Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Rivularia sp. PCC 7116 RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-21a(+)-LpxA

    Plasmid

    #12684

    Depositor

    SeWon Suh

    Article

    Lee et al Proteins. 2003 Nov 15. 53(3):772-4.

    Insert

    UDP-N-acetylglucosamine acyltransferase (HP1375 Helicobacter pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-28a-CylA

    Plasmid

    #48198

    Purpose

    Expresses CylA (fatty acyl-AMP ligase) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylA
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-PesaR-Dlux

    Plasmid

    #47657

    Purpose

    PesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-D
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added an additional esa box upstream of -35 site …

    Promoter

    PesaR-D

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only