We narrowed to 1,249 results for: vent

1. p406 – TDH3-Elo3-GFP11-Ura

   Plasmid

   #177734

   Purpose

   ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   Elo3 (ELO3 Budding Yeast)
   

   Use

   Tags

   GFP11 and HDEL

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX-SponGee-Kras

   Plasmid

   #134777

   Purpose

   mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

   Insert

   SponGee-Kras
   

   Use

   Tags

   FLAG and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Delta DED FLIP-L D/A

   Plasmid

   #13791

   Depositor

   Guy Salvesen

   Article

   Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

   Insert

   DELTA DED FLIP LONG (CFLAR Human)
   

   Use

   Tags

   8 x His tag

   Expression

   Bacterial

   Mutation

   Delta DED removes first 186 residues Asp to Ala m…

   Promoter

   Available since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHC004 (∆phaCAB)

   Plasmid

   #226198

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pPLN-hFluc-DM-HA-GFP11-KDEL

   Plasmid

   #177723

   Purpose

   DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFlucDM
   

   Use

   Tags

   GFP11, HA, KDEL, and Prolactin Signal Sequence

   Expression

   Bacterial and Mammalian

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. KG#797

   Plasmid

   #110916

   Purpose

   Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   unc-129 promoter
   STRN-1 (Sentryn)-GFP
   unc-54 3' control region with 1 intron just upstream and 1 intron in control region
   

   Use

   Tags

   GFP (contains 3 artificial introns)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIS-rPALcc

   Plasmid

   #145804

   Purpose

   expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed serine 767 to alanine to prevent glycosyl…

   Promoter

   CMV

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. KHBD00349

   Plasmid

   #34470

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5799 (dve Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCherryGFP-CoV2-Inframe-stop

   Plasmid

   #177620

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

    Plasmid

    #61629

    Purpose

    R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    mDII
    ntdTomato
    DII
    n3xVenus
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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