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We narrowed to 9,256 results for: SUB

Showing: 5751 - 5800 of 9256 results

  6. pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227900
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227900-plasmid-map-sequence-id-455749

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  7. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227901
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227901-plasmid-map-sequence-id-457382

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  8. pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227902
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227902-plasmid-map-sequence-id-453977

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  9. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227903
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227903-plasmid-map-sequence-id-452489

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  10. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227904
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227904-plasmid-map-sequence-id-452481

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  11. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227905
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227905-plasmid-map-sequence-id-456011

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  13. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Synthetic, Human, Photinus pyralis)
    Use
    Tags
    HA and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  14. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

    Plasmid
    #169835
    Purpose
    Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Substitution of amino acids F1308 - C1455 with a …
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169835-plasmid-map-sequence-id-333093

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  20. pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

    Plasmid
    #196617
    Purpose
    Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-BJ.1
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196617-plasmid-map-sequence-id-385178

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  21. Tet-inducible 3xFlag Mouse Caspase-9 ΔLS

    Plasmid
    #194609
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Domain
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Val 177 to Asp 353 (Casp9 Mouse)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Deletion of Large Subunit Domain eliminates bindi…
    Promoter
    CMV
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194609-plasmid-map-sequence-id-384892

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  22. Tet-inducible 3xFlag Mouse Caspase-9 ΔB LS

    Plasmid
    #194927
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Region Ala 264 to Asp 353
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Ala 264 to Asp 353 (Casp9 Mouse)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Deletion of Large Subunit Region Ala 264 to Asp 3…
    Promoter
    CMV
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194927-plasmid-map-sequence-id-384885

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  23. mGFP-hNET-A457P

    Plasmid
    #193365
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Harald Sitte
    Article
    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:
    Insert
    human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
    Use
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    A457P (GCC to CCC)
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193365-plasmid-map-sequence-id-381451

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  24. mGFP-hNET-R121A/K334A/R440A

    Plasmid
    #193366
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Harald Sitte
    Article
    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:
    Insert
    human Norepinephrine Transporter (SLC6A2 Human)
    Use
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193366-plasmid-map-sequence-id-381450

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  25. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid
    #184563
    Purpose
    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
    Depositor
    Kacper Rogala
    Article
    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:
    Insert
    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    All lysines were mutated to arginines. Codon-opti…
    Promoter
    CMV
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184563-plasmid-map-sequence-id-376975

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  28. pDR111_mannose_Klf4Gata-3

    Plasmid
    #188401
    Purpose
    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    Use
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188401-plasmid-map-sequence-id-370467

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  34. pVRC8400-SARS-CoV-2-S2P-B.1.621-AVI

    Plasmid
    #181694
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.621 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T95I, insert144T, Y144S, Y145N, R346K, E484K, N50…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181694-plasmid-map-sequence-id-354484

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  39. pVRC8400-SARS-CoV-2-S2P-C.37-AVI

    Plasmid
    #181693
    Purpose
    Expresses SARS-CoV-2 S2P protein from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-C.37 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G75V, T76I, R246N, ∆S247-∆D253, L452Q, F490S, D61…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181693-plasmid-map-sequence-id-354493

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  40. pVRC8400-SARS-CoV-2-RBD-SD1-N501Y-A570D-AVI

    Plasmid
    #176449
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing N501Y-A570D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-N501Y-A570D (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    N501Y, A570D
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176449-plasmid-map-sequence-id-347363

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  44. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-E484Q-AVI

    Plasmid
    #176455
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-L452R-E484Q (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L452R, E484Q
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176455-plasmid-map-sequence-id-347370

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  45. pVRC8400-SARS-CoV-2-RBD-K417T-E484K-N501Y-AVI

    Plasmid
    #176441
    Purpose
    Expresses SARS-CoV-2 RBD domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-K417T-E484K-N501Y (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176441-plasmid-map-sequence-id-347382

    Enlarge

  50. pVRC8400-SARS-CoV-2-RBD-K417N-L452R-T478K-AVI

    Plasmid
    #176447
    Purpose
    Expresses SARS-CoV-2 RBD domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-K417N-L452R-T478K (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417N, L452R, T478K
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176447-plasmid-map-sequence-id-347364

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Showing: 5751 - 5800 of 9256 results