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Showing: 2301 - 2320 of 7988 results

  3. Cwc25-pRSETA

    Plasmid
    #61043
    Purpose
    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.
    Depositor
    Nils Walter
    Article
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Cwc25
    Tags
    TEV site, Histidine Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pCRISPEY-GAL-Hyg-ADE2

    Plasmid
    #232104
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232104-plasmid-map-sequence-id-462310

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  7. pL0_AC-epyC

    Plasmid
    #202104
    Purpose
    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI sites fo…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202104-plasmid-map-sequence-id-405424

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  18. psiCHECK2 3'UTR KGA

    Plasmid
    #110402
    Purpose
    Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110402-plasmid-map-sequence-id-213697

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  20. eNS2B47NS3 S135A

    Plasmid
    #155318
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155318-plasmid-map-sequence-id-311249

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Showing: 2301 - 2320 of 7988 results