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pXPR_BRD003-sgCh2-2

Plasmid

#125767

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgCh2-4

Plasmid

#125768

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-4

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G563D

Plasmid

#116158

Purpose

Lentiviral expression of BRAF G563D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G563D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:deltaN-bmp4-pPH:vsvged

Plasmid

#111158

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

Insert

deltaNhBmp4 (BMP4 Human)

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Mutation

delta N, mutant BMP lacking the N terminus (delta…

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2IP_p.I979M

Plasmid

#81568

Purpose

Gateway Donor vector containing ERBB2IP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2IP (ERBIN Human)

Use

Gateway entry vector

Mutation

T271P; D547G; I979M; E1205G; Q1226P; K1287R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-active WRN

Plasmid

#125788

Purpose

constitutive, ectopic expression of human active WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-active WRN (WRN Human)

Tags

5x myc

Expression

Mammalian

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A K577M

Plasmid

#125791

Purpose

constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A/K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A/K577M (exonuclease/helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-1

Plasmid

#125776

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-1 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-2

Plasmid

#125777

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-2 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN-EIJ

Plasmid

#125774

Purpose

constitutive expression of a guide RNA targeting an exon-intron junction of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN-EIJ (WRN Human)

Use

CRISPR

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Theta [L123/46R]

Plasmid

#188158

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Theta (Human). Derived from hybridoma L123/46

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Theta (Homo sapiens) recombinant mouse monoclonal antibody (GABRQ Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5-FLAG-MKK6-K82M

Plasmid

#21583

Depositor

John Kyriakis

Article

Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

Insert

MKK6 (MAP2K6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lysine 82 to methionine

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pXPR_BRD003-sgPOLR2D

Plasmid

#125769

Purpose

constitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgPOLR2D (POLR2D Human)

Use

CRISPR

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v5 (B.1.617.3)

Plasmid

#212540

Purpose

Encodes SARS-CoV-2 spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 AY.4.2

Plasmid

#212457

Purpose

Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 AY.1

Plasmid

#212433

Purpose

Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 AY.2

Plasmid

#212434

Purpose

Encodes SARS-CoV-2 spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v2 (B.1.617.1 v2)

Plasmid

#212429

Purpose

Encodes SARS-CoV-2 spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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