We narrowed to 19,524 results for: ACE;

1. pSG81-pLas-tetO-deGFPssrA

   Plasmid

   #102454

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Use

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   pLas-tetO

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA231-CRISPR

   Plasmid

   #138712

   Purpose

   Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL498

   Plasmid

   #169154

   Purpose

   Encodes OptoQ-INVRT4

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRi-D2

   Plasmid

   #182927

   Purpose

   inducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803

   Depositor

   Himadri Pakrasi

   Article

   Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

   Insert

   ddcpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscMS2(WTx2)-EGFP

   Plasmid

   #140258

   Purpose

   MS2CP-dependent translational repression

   Depositor

   Hirohide Saito

   Article

   Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

   Insert

   Two MS2CP-binding sites embedded in scaffold
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL30009

   Plasmid

   #50301

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   N terminal Myc tag (4x Myc)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET11a-STEPtag

   Plasmid

   #159373

   Purpose

   Expresses STEPtag in E. coli

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   STEPtag
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 130_pSKB3-LUMP-P.leioghnati

   Plasmid

   #65894

   Purpose

   Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Insert

   LUMP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PclpB GFP pZa

   Plasmid

   #170074

   Purpose

   GFP expression under the control of E. coli clpB promoter

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PclpB

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-T7-Bottom-T

    Plasmid

    #87310

    Purpose

    Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Bottom" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHES822

    Plasmid

    #89193

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-C1-mCherry-LOVPLD

    Plasmid

    #219559

    Purpose

    ER-targeted LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    C1-mCherry-LOVPLD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJYScr

    Plasmid

    #132719

    Purpose

    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

    Depositor

    Jan Marienhagen

    Article

    Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICSL50009

    Plasmid

    #50309

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal HA tag (6x Human influenza hemagglutinin)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMAL724

    Plasmid

    #169151

    Purpose

    Encodes OptoQ-AMP5

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. vYM014_phiC31-nTRE-Ins(low)-pEF1s(low)-H2b-Citrine

    Plasmid

    #197682

    Purpose

    "Insert(low)-pEF1s(low)" reporter plasmid

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    pEF1s-H2B-Citrine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC-EsaR-D91G

    Plasmid

    #47646

    Purpose

    pAC-EsaR with a a272g mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-D91G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 91 to Glycine

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5/FRT/TO_Lifeact_STf

    Plasmid

    #182011

    Purpose

    CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lifeact_STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pscPP7(2xWT)-EGFP

    Plasmid

    #140261

    Purpose

    PP7CP-dependent translational repression

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    Two PP7CP-binding sites embedded in scaffold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-deltaCKAR

    Plasmid

    #31526

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Delta-selective C kinase activity reporter
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only