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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1

Plasmid

#32466

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

Expression

Bacterial

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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Lapronic/pRSETB

Plasmid

#140755

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Arch-EEQ

Plasmid

#45188

Purpose

Arch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95N

Depositor

Mark Schnitzer

Article

Gong et al PLoS One. 2013 Jun 19;8(6):e66959.

Insert

enhanced Archaerhodopsin

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Mutation

D95Q, D106E

Promoter

CamKIIa

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

Plasmid

#198599

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-143-144

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo6

Plasmid

#203957

Purpose

Cloning plasmid containing the kanamycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Kanamycin Resistance Gene

Expression

Plant

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M4NS_AmpR

Plasmid

#119156

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano30/pcDNA3

Plasmid

#51963

Purpose

mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano30

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pMRMTK-clo10

Plasmid

#203961

Purpose

Cloning plasmid containing the erythromycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Erythromycin Resistance Gene

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Tt-NylC_pET28a

Plasmid

#215431

Purpose

Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Tt-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2T SHP1 CS

Plasmid

#8595

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Mutation

C453S (catalytically inactive)

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111926

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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OeNL(Ca2+)_18μ-pcDNA3

Plasmid

#111928

Purpose

Orange color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

OeNL(Ca2+)_18μ

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111927

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M7NS_AmpR

Plasmid

#119157

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTorPE-FRISZ

Plasmid

#176888

Purpose

Far-red fluorescent zinc indicator FRISZ in pTorPE vector

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Tags

Periplasmic expression TorA tag and Twin-Strep-ta…

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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