We narrowed to 10,458 results for: T7

1. 6_T7-Lyn-mScarlet3

   Plasmid

   #225933

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Lyn membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   Lyn-mScarlet3
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 7_T7-Src64B-mScarlet3

   Plasmid

   #225934

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Src64B membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   Src64B-mScarlet3
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 8_T7-PH-mScarlet3

   Plasmid

   #225935

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   PH-mScarlet3
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 9_T7-mScarlet3-LactC2

   Plasmid

   #225936

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-LactC2
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7CFE1_EVA71_VP12A_C110A

   Plasmid

   #212976

   Purpose

   Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Enterovirus A71 4643 VP12A C110A
   

   Use

   Mammalian cell free protein expression

   Tags

   HA tag

   Expression

   Mutation

   changed cysteine 110 from 2A to alanine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. RFXANK:JMC029-A02:C213521

   Plasmid

   #183980

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   RFXANK (RFXANK Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. MPP1:HPC09T-D06:C210522

   Plasmid

   #183976

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   MPP1 (MPP1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pmCellFree_KA1_SARS-COV-2_ORF7b

   Plasmid

   #169394

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   T7- CMV/CAG

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-Pur-AktAR2-D

   Plasmid

   #125196

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Insert

   AktAR2-D
   

   Use

   Transposon

   Tags

   6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACY.HR3_T7_term_HR4

    Plasmid

    #111985

    Purpose

    Subcloning vector position 4

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACY.HR4_T7_term_HRR

    Plasmid

    #111986

    Purpose

    Subcloning vector position 5

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pACY.HR2_T7_term_HR3

    Plasmid

    #111984

    Purpose

    Subcloning vector position 3

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACY.HR1_T7_term_HR2

    Plasmid

    #111983

    Purpose

    Subcloning vector position 2

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACY.HRF_T7_term_HR1

    Plasmid

    #111981

    Purpose

    Sub cloning vector position 1

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET11a asyn Q79N Q109N (TAC-->TAT)

    Plasmid

    #105738

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q79N/Q109N

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 2QNR (NEDD5)

    Plasmid

    #101273

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NEDD5 (SEPTIN2 Human)
    

    Use

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pJK103

    Plasmid

    #73265

    Purpose

    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)

    Depositor

    T. J. Kappock

    Insert

    hypothetical acetic acid resistance protein B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. KCTD17

    Plasmid

    #74661

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KCTD17A (KCTD17 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pJK610

    Plasmid

    #72531

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK540

    Plasmid

    #72446

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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