We narrowed to 159,428 results for: addgene

1. lenti-SpCas9 hygro

   Plasmid

   #104995

   Purpose

   This lentiviral construct delivers hSpCas9 and hygromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

   Plasmid

   #135014

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   126aa domain from HSV-1 UL12 fused to the C-termi…

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

   Plasmid

   #135013

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   126aa domain from HSV-1 UL12 fused to the N-termi…

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiGuide-chr20-49345720-gRNA1

   Plasmid

   #232623

   Purpose

   Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

   Depositor

   Claes Wadelius

   Article

   Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiGuide-chr20-49345720-gRNA2

   Plasmid

   #232624

   Purpose

   Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

   Depositor

   Claes Wadelius

   Article

   Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiGuide-chr20-49345720-gRNA3

   Plasmid

   #232625

   Purpose

   Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

   Depositor

   Claes Wadelius

   Article

   Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid

    #201677

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-3g-EEA-2g-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBP-HsSyx3

    Plasmid

    #179287

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMBP-HsSyx-375-792

    Plasmid

    #179291

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 375-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGK-Ldb3-mKate2

    Plasmid

    #128766

    Purpose

    Fluorescent mKate reporter for Ldb3

    Depositor

    Deepak Srivastava

    Article

    Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

    Insert

    Ldb3 promoter (Ldb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ldb3

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS418

    Plasmid

    #11256

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAG pShuttle

    Plasmid

    #13427

    Depositor

    Ronald Kahn

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6 pShuttle

    Plasmid

    #13428

    Depositor

    Ronald Kahn

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS428

    Plasmid

    #11257

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTN186

    Plasmid

    #34890

    Depositor

    Mitsuyasu Hasebe

    Type

    Empty backbone

    Use

    Moss targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

19. FUdGW-Tomato

    Plasmid

    #22771

    Depositor

    Konrad Hochedlinger

    Insert

    DsRed
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGKFlpebpA

    Plasmid

    #13441

    Depositor

    Philippe Soriano

    Insert

    PGKFlpebpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge