We narrowed to 13,713 results for: cas9

1. pCMV-AAAAA-nCas9(D10A)-UGI

   Plasmid

   #187409

   Purpose

   DddA AAAAA variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA AAAAA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-cGAS g2 Cas9-T2A-mCherry

   Plasmid

   #164253

   Purpose

   Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgcGAS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

   Plasmid

   #186699

   Purpose

   Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-cmv-SpCas9-TadCBEa-BlastR

   Plasmid

   #193847

   Purpose

   Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

   Plasmid

   #112719

   Purpose

   An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Mecp2

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

   Plasmid

   #159920

   Purpose

   Mutagenesis of Vamp2

   Depositor

   Larry Zweifel

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZac2.1 SV40-CMV-SaCas9-3xNLS

   Plasmid

   #78601

   Purpose

   AAV vector containing SaCas9

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   SV40-CMV-SaCas9-3xNLS
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40, CMV promoters

   Available since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

10. Tol2-LyzC-Cas9-pA-Cry-GFP

    Plasmid

    #168239

    Purpose

    "neutrophil specific cas9 expression in zebrafish with green lens"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Cas9
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGinB-8X(GGS)-dCas9-FLAG-NLS

    Plasmid

    #81205

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

    Plasmid

    #163552

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-cGAS g3 Cas9-T2A-mCherry

    Plasmid

    #164254

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SMVP-Cas9N-U6-gRNA TdR

    Plasmid

    #80940

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

    Plasmid

    #71216

    Purpose

    dCas9 fused to human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A catalytic domain
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pspCas9-3'UTR-ST2-com-vector

    Plasmid

    #136269

    Purpose

    Expresses SpCas9 and sgRNA Scaffold

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Insert

    SpCas9 and sgRNA scaffold
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. G1397 DddAtox-C–SaKKH-Cas9(D10A)

    Plasmid

    #157840

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458 pSp Cas9 Rhino1 tagA

    Plasmid

    #211627

    Purpose

    Cas9 Rhino1 tagA

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    RHNO tagA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSaCas9-sgRNA-Tetra-com vector

    Plasmid

    #131227

    Purpose

    For expressing gRNA, whose Tetraloop is replaced with a com aptamer

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    vector for com modified sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKMD111e - intein-Cas9(S219-G521R)

    Plasmid

    #64192

    Purpose

    Expresses intein-Cas9(S219-G521R) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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