We narrowed to 4,241 results for: pes;

1. pCDH-Regnase-3 WT-T2A-copGFP

   Plasmid

   #222660

   Purpose

   Lentiviral vector to express mouse Regnase-3 WT

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12c (Zc3h12c Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   mutated stop codon prior to a T2A peptide

   Promoter

   EF1α

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-Regnase-1 D141N

   Plasmid

   #222654

   Purpose

   Mammalian expression vector to express mouse Regnase-1 D141N mutant

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Expression

   Mammalian

   Mutation

   mutated aspartic acid 141 to asparagine to abroga…

   Promoter

   CMV

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57 mini-FLAG-HA-Regnase-3 D252N

   Plasmid

   #222652

   Purpose

   For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12c (Zc3h12c Mouse)
   

   Use

   In vitro transcription

   Tags

   FLAG and HA

   Mutation

   mutated aspartic acid 252 to asparagine to abroga…

   Promoter

   T7

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

   Plasmid

   #222649

   Purpose

   For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   In vitro transcription

   Tags

   FLAG and HA

   Promoter

   T7

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_ALX4-Flag

   Plasmid

   #215029

   Purpose

   Mammalian expression of human ALX4

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   ALX homeobox 4 (ALX4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_ALX4-Flag-HA

   Plasmid

   #215028

   Purpose

   Mammalian expression of human ALX4

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   ALX homeobox 4 (ALX4 Human)
   

   Tags

   Flag-HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas_4(I-U)/1_2_VB

   Plasmid

   #186445

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Mutation

   Cas4 domain swapped with Cas4 domain from G. sulf…

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATX3-JD

   Plasmid

   #184247

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 Josephin domain (ATXN3 Human)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Premature stop codon V183X

   Promoter

   T7 promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJET-col2a1a-KI-donor

   Plasmid

   #184876

   Purpose

   Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair

   Depositor

   Christoph Winkler

   Article

   Hui et al Development. 2022 May 20. pii: 275483. d

   Inserts

   Collagen2a1a 5' homology arm
   Collagen2a1a 3' homology arm
   p2a-CreERT2
   Myosin light chain 2 promoter
   EGFP
   

   Use

   CRISPR and Cre/Lox

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-GFP11-RhoA

    Plasmid

    #181860

    Purpose

    Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    GFP11-RhoA (RHOA Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 330-BFP-hCYP3A4-enhancer-R-sgRNA

    Plasmid

    #176841

    Purpose

    Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer right boundary
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 330-cherry-hCYP3A4-enhancer-L-sgRNA

    Plasmid

    #176840

    Purpose

    Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer left boundary
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac1_GI.1_A37C-A44C

    Plasmid

    #162582

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Alanine 37 changed to Cysteine and Alanine 44 cha…

    Promoter

    polyhedring

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac1_GI.1_Q141V-P221L

    Plasmid

    #162584

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Glutamine 141 changed to Valine and Proline 221 c…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1_GI.1_L144C-P221C

    Plasmid

    #162585

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Leucine 144 changed to Cysteine and Proline 221 c…

    Promoter

    polyhderin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac1_GI.1_G131C-N172C

    Plasmid

    #162586

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Glycine 131 changed to Cysteine and Asparagine 17…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1_GI.1_N167C-L169C

    Plasmid

    #162587

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Asparagine 167 changed to Cysteine and Leucine 16…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/TO-BcRF1-ORF24-2xStrep

    Plasmid

    #138454

    Purpose

    Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    BcRF1 amino acids 1-178 fused to ORF24 amino acid…

    Promoter

    CMV

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO-UL87-ORF24-2xStrep

    Plasmid

    #138455

    Purpose

    Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87-ORF24 (UL87, ORF24 HCMV/KSHV)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    UL87 amino acids 1-248 fused to ORF24 amino acids…

    Promoter

    CMV

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. rd

    Plasmid

    #112912

    Purpose

    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag

    Depositor

    C. James McKnight

    Article

    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

    Insert

    dematin (DMTN Human)
    

    Tags

    Glutatione-S-Tranferase and precision protease si…

    Expression

    Bacterial

    Mutation

    PEST sequence near the N-terminal removed. 5′-GCG…

    Promoter

    T7

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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