We narrowed to 8,683 results for: sgRNA

1. LRCherry2.1

   Plasmid

   #108099

   Purpose

   Lentiviral expression plasmid of sgRNA with mCherry

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CMV-NLS-dCas9-NLS

   Plasmid

   #99908

   Purpose

   For the expression of NLS-dCas9-NLS

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-dCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRG2.1

   Plasmid

   #108098

   Purpose

   Lentiviral expression plasmid of sgRNA with GFP

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-EN1201

   Plasmid

   #92144

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
   

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGCP123-EbpA_g1

   Plasmid

   #153517

   Purpose

   sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRG2.1_Puro

   Plasmid

   #125594

   Purpose

   Lentiviral expression of sgRNA with GFP and puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPRi_Mxi1_yl

   Plasmid

   #91248

   Purpose

   CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAT009

    Plasmid

    #171635

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Sg-A

    Plasmid

    #83807

    Purpose

    Express sgRNA targeting SA site

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBBR_Cas9_sp1

    Plasmid

    #154851

    Purpose

    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

    Depositor

    John van der Oost

    Article

    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

    Inserts

    Spcas9 gene
    sgRNA scaffold
    

    Use

    CRISPR

    Tags

    6xHis

    Mutation

    Codon harmonized variant for expression in Rhodob…

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-EN479

    Plasmid

    #86234

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse rosa26 locus
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJE53

    Plasmid

    #194153

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAT005

    Plasmid

    #171632

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-a and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pspCas9-HF-tetra-com-vector

    Plasmid

    #132555

    Purpose

    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Expression

    Mammalian

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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