We narrowed to 4,230 results for: Pet2

1. pET28a-PTBP1-RRM12CCtoSS

   Plasmid

   #89155

   Purpose

   Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
   

   Use

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-p53 (1-73) (72R)

   Plasmid

   #62082

   Purpose

   Expression of human p53 (1-73) (72R) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72R

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-Hook3 aa 1-239

   Plasmid

   #74609

   Purpose

   Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b+R3(R112C/T180C)

   Plasmid

   #108195

   Purpose

   Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA3(R112C/T180C) fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28 mSmo-Cterm

   Plasmid

   #58703

   Purpose

   used for anti-mouse Smoothened antibody production (for affinity purification)

   Depositor

   Matthew Scott

   Article

   Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

   Insert

   Smo (Smo Mouse)
   

   Use

   Tags

   6X-HIS

   Expression

   Bacterial

   Mutation

   contains C terminus (aa 543-793)

   Promoter

   Available since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET29b-Ava3858-3857

   Plasmid

   #45744

   Depositor

   Christopher T Walsh, Emily Balskus

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3857
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET28b Me31B-3Myc

   Plasmid

   #113001

   Purpose

   For protein expression and purification of Myc-tagged full-length Drosophila me31B

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length me31B (me31B Fly)
   

   Use

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET20b-delta133HtrA2 S400D

   Plasmid

   #16156

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S400D

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pET29NK-LGK/mGFPmut3

   Plasmid

   #81168

   Purpose

   GFP fusion to levoglucosan kinase

   Depositor

   Timothy Whitehead

   Article

   Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

   Insert

   Levoglucosan kinase wild-type
   

   Use

   Tags

   His6x and mGFPmut3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET20b-delta133HtrA2 G399S

    Plasmid

    #16158

    Depositor

    L. Miguel Martins

    Article

    Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    G399S

    Promoter

    T7

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only