We narrowed to 7,129 results for: Trac;

1. pTBL514 TG sgRNA

   Plasmid

   #126440

   Purpose

   To target a protospacer with TG at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with TG at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL513 TT sgRNA

   Plasmid

   #126441

   Purpose

   To target a protospacer with TT at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with TT at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL322 GC

   Plasmid

   #126950

   Purpose

   To target a protospacer with GC at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with GC at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTBL581 GA

   Plasmid

   #126951

   Purpose

   To target a protospacer with GA at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with GA at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL601 AG

   Plasmid

   #126952

   Purpose

   To target a protospacer with AG at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with AG at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTBL602 CG

   Plasmid

   #126953

   Purpose

   To target a protospacer with CG at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with CG at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-4T-3-cytXlATL2-R232Q

   Plasmid

   #124064

   Purpose

   Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXlATL2-R232Q
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-kanMX6-P3nmt1-mECitrine

   Plasmid

   #105151

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

   Insert

   kanMX6-P3nmt1-mECitrine
   

   Use

   Tags

   3nmt1-mECitrine

   Expression

   Bacterial and Yeast

   Mutation

   F64L, Q69M, A206K

   Promoter

   3nmt1

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH009

   Plasmid

   #80399

   Purpose

   Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   3xFLAG-Venus YFP-AAV
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal LZx domain and N-terminal 3xFLAG tag, …

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVH015

    Plasmid

    #80398

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30-SBL1(160S,HisTagDel)

    Plasmid

    #66880

    Purpose

    Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (S160S)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30-SBL1(160E,HisTagDel)

    Plasmid

    #66879

    Purpose

    Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (S160E)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV10

    Plasmid

    #63213

    Purpose

    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPCX mito Grx1-roGFP2

    Plasmid

    #64977

    Purpose

    Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a_6H-TAQ_E602D

    Plasmid

    #166944

    Purpose

    Bacterial expression of Taq DNA polymerase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    Thermus aquaticus DNA polymerase
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    E602D

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-gRNA

    Plasmid

    #158973

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    gRNA3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hBACCS2-IRES-mCherry

    Plasmid

    #72892

    Purpose

    Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    hBACCS2-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-trGluc

    Plasmid

    #158959

    Purpose

    DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    trGluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PEmaxNG-_2star_IVT

    Plasmid

    #227678

    Purpose

    plasmid for SpCas9-NG-PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    NG-PEmax_2_star
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP

    Plasmid

    #232754

    Purpose

    Expresses EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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