We narrowed to 2,783 results for: aen

1. NgΔIQ FUNgDIQGW

   Plasmid

   #92232

   Purpose

   Lentiviral expression of human Ng with deletion mutation

   Depositor

   Weifeng Xu

   Article

   Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

   Insert

   Ng (NRGN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta IQ

   Promoter

   hUbC

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

   Plasmid

   #85583

   Purpose

   cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

   Depositor

   Paul Sternberg

   Article

   Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   Prab-3-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Prab-3-GAL4-SK(DBD)-VP64

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS145

   Plasmid

   #179159

   Purpose

   Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

   Depositor

   Iva Greenwald

   Article

   Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Insert

   rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Flexon replacing 2nd intron of 2xNLS::GFP

   Promoter

   rps-27p

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMT3-FBF2

   Plasmid

   #166972

   Purpose

   Expresses FBF-2 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ150-GFP(dpiRNA)::CDK-1

   Plasmid

   #107938

   Purpose

   cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   cdk-1

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

   Plasmid

   #47550

   Purpose

   Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   ttTi5605 sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET_SZ1-RGG-GFP-RGG

   Plasmid

   #124937

   Purpose

   Expression of SZ1-RGG-GFP-RGG

   Depositor

   Benjamin Schuster, Matthew Good, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159857

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159814

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPReporter-mCherry

    Plasmid

    #65008

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    User-defined

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT3024

    Plasmid

    #122542

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_mt::CemOrange2
    

    Use

    Tags

    MLSLRQSIRFFKPATRTLCSSRTLL

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN HA-NDP52

    Plasmid

    #199307

    Purpose

    For expression of the mammalian NDP52 tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH4169

    Plasmid

    #200108

    Purpose

    Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    snb-1 (snb-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Prig-3

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159813

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159850

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEY26

    Plasmid

    #191031

    Purpose

    cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    cho-1(3.3-2.6k) (cho-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEY39

    Plasmid

    #191043

    Purpose

    acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(2.1k) (acr-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA_SZ1-RGG-GFP-RGG

    Plasmid

    #124932

    Purpose

    Expression of SZ1-RGG-GFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

    Plasmid

    #130275

    Purpose

    Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    Arch(D95N)
    

    Use

    Tags

    2x Myc-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pv6_MBD1_MBD

    Plasmid

    #179404

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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