We narrowed to 5,111 results for: codon optimized

1. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

   Plasmid

   #188975

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g1 guide RNA
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

   Plasmid

   #188976

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g2 guide RNA
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJF152_Phsp_Cas9

   Plasmid

   #163862

   Purpose

   heat shock-induced expression of Cas9 in C. elegans

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   Cas9, C. elegans codon optimized with intron and SV40_NLS
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   Phsp-16.48

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-T3-HypaCAS9-pA

   Plasmid

   #131467

   Purpose

   Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

   Insert

   Codon optimized HypaCas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1146-pCSDest-AcrIIC3Nme-mCherry

   Plasmid

   #126202

   Purpose

   Mammalian expression of AcrIIC3Nme fused to mCherry

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Insert

   codon-optimized AcrIIC3Nme
   

   Tags

   FLAG/NLS and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-SERTN177E-myc

   Plasmid

   #107474

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Mutation

   R596Q

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-SERTY289I-myc

   Plasmid

   #107480

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y289I with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Mutation

   N177E

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-SERTK37Q-myc

   Plasmid

   #107476

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K37Q approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Mutation

   K37Q

   Promoter

   CMV

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-SERTL406I-myc

   Plasmid

   #107487

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L406I with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Mutation

   L406I

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-SERTE392M-myc

    Plasmid

    #107486

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E392M with approximately wildtype activity

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    E392M

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-SERTT233F-myc

    Plasmid

    #107481

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T233F with approximately wildtype activity

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    T233F

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-SERTI576V-myc

    Plasmid

    #107473

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transport

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    I576V

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-SERTL550V-myc

    Plasmid

    #107472

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L550V approximately wildtype transport

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    L550V

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pfMDH_K381A

    Plasmid

    #67732

    Purpose

    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 381 to alanine

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pfMDH_N300D

    Plasmid

    #67728

    Purpose

    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to aspartate

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pfMDH_R373A

    Plasmid

    #67731

    Purpose

    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed arginine 373 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pfMDH_N300S

    Plasmid

    #67729

    Purpose

    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to serine

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. miABE

    Plasmid

    #205412

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Human, Codon optimised)
    

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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