We narrowed to 25,015 results for: lars

1. pAPInt-GFP-G418

   Plasmid

   #236478

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONOR 6colors

   Plasmid

   #229974

   Purpose

   bigMamAct assembled plasmid for 6colors cellular labelling

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBTK1061

   Plasmid

   #209550

   Purpose

   BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA gene

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al bioRxiv 2023.09.19.558440

   Insert

   E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rp51a Hyperstabilized 5'SS

   Plasmid

   #100988

   Purpose

   pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   5'ss mut ATG/GTATGT -> AAG/GTAAGT

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. RP51A-NoBS-NoPseudoBS

   Plasmid

   #100986

   Purpose

   RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTALE-STAR-T_RFP entry

   Plasmid

   #87526

   Purpose

   Destination vector for domain insertion

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale empty functiona…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTALE-STAR-A_RFP entry

   Plasmid

   #87527

   Purpose

   Destination vector for domain insertion

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale empty functiona…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTALE-STAR-C_RFP entry

   Plasmid

   #87528

   Purpose

   Destination vector for domain insertion

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale empty functiona…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTALE-STAR-G_RFP entry

   Plasmid

   #87529

   Purpose

   Destination vector for domain insertion

   Depositor

   Steve Pollard

   Article

   Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology and TALEN; Tale empty functiona…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NW11

    Plasmid

    #133443

    Purpose

    Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NW9

    Plasmid

    #133442

    Purpose

    Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. circRNA-synIRES-R25-NanoLuc

    Plasmid

    #188116

    Purpose

    Template plasmid to transcribe circular RNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

    Plasmid

    #126049

    Purpose

    For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PP7_mCherry

    Plasmid

    #61763

    Purpose

    Expresses PP7 coat protein fused to mCherry

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    PP7 coat protein fused to mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) CircRNA Mini Vector

    Plasmid

    #60648

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CH-GECO2.1

    Plasmid

    #52099

    Purpose

    mCherry-based Calcium indicators

    Depositor

    Robert Campbell

    Article

    Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

    Insert

    CH-GECO2.1
    

    Use

    Tags

    6 His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD promotor

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only