We narrowed to 2,376 results for: pET28

Showing: 2341 - 2360 of 2376 results

Results per page:

Sort results by:

CMV-VPR-S-L1-dCjCas9 MiniCAFE

Plasmid

#169910

Purpose

Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Expression

Mammalian

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHis-BRD4 BD1

Plasmid

#196544

Purpose

Expression of BRD4 Bromodomain (BD1) in E.coli

Depositor

Byung Il Lee

Article

Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

Insert

BRD4 BD1 (BRD4 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-EGFP-PH-PLCD1

Plasmid

#183675

Purpose

PtdIns(4,5)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLCD1 (PLCD1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 11-140

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Pdpy-30-miniCAFE

Plasmid

#170115

Purpose

Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

Dpy-30

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG87 FAST

Plasmid

#130719

Purpose

Expresses His-tagged FAST (also called YFAST) in bacteria

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

FAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Met1 diUb (1-152)

Plasmid

#110766

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
H6SUMO-IntC-P53(304-393)

Plasmid

#200316

Purpose

bacterial expression of P53 TET-CTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Tags

His6-SUMO and designed intein C

Expression

Bacterial

Promoter

T7

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-SMT3-SopB

Plasmid

#183676

Purpose

Recombinant Salmonella Typhimurium SopB

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)

Tags

His6-S. cerevisiae SMT3(2-101)

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
His-GINA3

Plasmid

#74980

Purpose

Expresses His tagged GINA3 protein in bacteria.

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )

Tags

His

Expression

Bacterial

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXB4 (human) HIS-tag pET new RBS

Plasmid

#8525

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Contains improved Ribosomal binding site; makes …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSN554

Plasmid

#177361

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

Leucine zipper, StrepII tag, and mScarlet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ppil2.280.457.Y389H

Plasmid

#137658

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389H

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ppil2.280.457.Y389W

Plasmid

#137657

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389W

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
WT USP7 TRAF

Plasmid

#227226

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 62-205

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mature ISG15 C-term. domain (79-157)

Plasmid

#110764

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
proISG15 C-term. domain (79-165)

Plasmid

#110763

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge