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pBabe-Puro-IKBalpha-mut (super repressor)

Plasmid

#15291

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Mutation

S32A/S36A: “super-repressor” allele (resistant to…

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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p4455 FLAG-hPLIC-2

Plasmid

#8661

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pLJM5-WT PHGDH

Plasmid

#83901

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NfiB

Plasmid

#64900

Purpose

Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

NfiB (Nfib Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

Plasmid

#233217

Purpose

Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv 2023.10.24.563674

Insert

Anti-amyloid-β 40/42 nanobody (R3VQ)

Tags

Sortase tag, His tag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-403-505

Plasmid

#165095

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-SMS2-TEV-Twin-Strep

Plasmid

#202529

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

SGMS2 (SGMS2 Human)

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted stop codon (TGA)

Promoter

CAG and chicken β-actin promoter

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTKRED

Plasmid

#41062

Depositor

Edward Cox, Thomas Kuhlman

Article

Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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