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pBX_RPS2_smGFP_V5

Plasmid

#236088

Purpose

Constitutively encodes RPS2-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting the 40S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPS2-smGFP-V5 (RPS2 Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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HA Depdc5 pRK5

Plasmid

#46327

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

HA

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pCI-Myc3-HPS4

Plasmid

#133931

Purpose

Expresses 3xMyc-HPS4 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

Insert

HPS4 (HPS4 Human)

Tags

3xMyc tag

Expression

Mammalian

Mutation

Glutamic acid 229 to Glycine; Valine 552 to Methi…

Promoter

CMV I.E.

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pEF019 MBP-β-catenin-His

Plasmid

#154070

Purpose

Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CTNNB1 (CTNNB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (1-1304)

Plasmid

#74244

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-1304

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Synthetic, Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp8-EGFP

Plasmid

#165113

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp8 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Flag Leucyl tRNA synthetase pRK5

Plasmid

#46341

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

leucyl tRNA synthetase (LARS1 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only