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Showing: 4721 - 4760 of 8321 results

  1. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

    Plasmid
    #44081
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44081-plasmid-map-sequence-id-61675

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  2. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid
    #104419
    Purpose
    transient expression of dCas9-dp300 fusion protein
    Depositor
    Stephan Beck
    Insert
    dp300
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    S1396R-S1397R
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104419-plasmid-map-sequence-id-204303

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  3. pSpot-Tag-Lamin

    Plasmid
    #117062
    Purpose
    For mammalian expression of lamin B1 with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Tags
    Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117062-plasmid-map-sequence-id-224387

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  4. pENTRY4_HuD

    Plasmid
    #65754
    Purpose
    entry vector with HuD
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65754-plasmid-map-sequence-id-114982

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  7. pPyCAG-Nanog(K140A)-IP

    Plasmid
    #62998
    Purpose
    Expresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K140A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 140 (141 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62998-plasmid-map-sequence-id-104601

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  8. pIC84

    Plasmid
    #63062
    Purpose
    Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    hCENP-H, IMAGE clone 3451767 (CENPH Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63062-plasmid-map-sequence-id-104377

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  9. pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

    Plasmid
    #44093
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44093-plasmid-map-sequence-id-61671

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  10. pLV H2afy-V5

    Plasmid
    #175122
    Purpose
    Lentiviral expression of mouse Macroh2a1-V5
    Depositor
    Larry Gerace
    Insert
    Macroh2a1 (Macroh2a1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175122-plasmid-map-sequence-id-351930

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  11. H2B-sfGFP-T2A-iCre

    Plasmid
    #178546
    Purpose
    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox and Mouse Targeting; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178546-plasmid-map-sequence-id-360157

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  12. p2Bac.Flag.p34

    Plasmid
    #39221
    Depositor
    Aziz Sancar
    Insert
    p34 (GTF2H3 Human)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Pph
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV asyn S129E

    Plasmid
    #36068
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129E (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S129E
    Promoter
    CMV
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  14. pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

    Plasmid
    #44070
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44070-plasmid-map-sequence-id-61679

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  15. miniSOG-Farnesyl-5

    Plasmid
    #62782
    Purpose
    Localization: Membrane, Excitation:448 / 473, Emission:500 / 528
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    miniSOG
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62782-plasmid-map-sequence-id-254842

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  16. pBluescript alpha-SSeCKS

    Plasmid
    #61295
    Purpose
    contains rat alpha SSeCKS cDNA
    Depositor
    Irwin Gelman
    Insert
    alpha SSeCKS (Akap12 Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cDNA with 1kb of 3' UTR and 150bp of 5'…
    Promoter
    T7
    Available since
    Dec. 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  18. TH

    Plasmid
    #39079
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TH (TH Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. Hs_MSI2_RBD

    Plasmid
    #61113
    Purpose
    Express His tagged human MSI2 RNA binding domain
    Depositor
    Sean Ryder
    Insert
    MSI2 RNA binding domain (MSI2 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61113-plasmid-map-sequence-id-99120

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  20. pTF280 (pFA6a-PSc-6xGly-2xStrep-HphMX)

    Plasmid
    #44076
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44076-plasmid-map-sequence-id-61685

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  21. 13SA-csy4

    Plasmid
    #97037
    Purpose
    Spectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectors
    Depositor
    Chris Jeans
    Insert
    Cys4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97037-plasmid-map-sequence-id-182491

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  22. gamma1-EGFP

    Plasmid
    #119224
    Purpose
    GABAA receptor expression (gamma 1 subunit with EGFP tag at the C-terminus)
    Depositor
    Angel de Blas
    Insert
    GABRG1 (Gabrg1 Rat)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119224-plasmid-map-sequence-id-234650

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  23. Lazarus Gata 3 flag

    Plasmid
    #80135
    Purpose
    retroviral expression of Gata3-Flag
    Depositor
    Ellen Rothenberg
    Insert
    GATA binding protein 3 (Gata3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    80135-plasmid-map-sequence-id-157357

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  24. pDON-AI-baIaa

    Plasmid
    #51474
    Purpose
    Express mouse TH2B and TH2A in mammalian cells
    Depositor
    Shunsuke Ishii
    Inserts
    histone cluster 1, H2aa (H2ac1 Mouse)
    histone cluster 1, H2ba (H2bc1 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51474-plasmid-map-sequence-id-78546

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  25. pcDNA3.1-3xHA-mASL

    Plasmid
    #34577
    Purpose
    NOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.
    Depositor
    Peter Murray
    Insert
    ASL (Asl Mouse)
    Use
    Tags
    1xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    34577-plasmid-map-sequence-id-265171

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  26. pHH0103 IQGAP1 WW domain

    Plasmid
    #104279
    Purpose
    Bacterial expression of WW domain from IQGAP1
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    IQGAP1 WW domain (IQGAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104279-plasmid-map-sequence-id-203685

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  27. PTPRO

    Plasmid
    #38969
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRO (PTPRO Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. pUASp YFP Cdc42 CA

    Plasmid
    #52250
    Purpose
    Drosophila expression of YFP-Cdc42 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pPyCAG-Nanog(L122A)-IP

    Plasmid
    #62992
    Purpose
    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog L122A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Leucine 122 (123 AA in Mouse Protein) to …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62992-plasmid-map-sequence-id-104441

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  32. COVID-SARS2 NSP10

    Plasmid
    #159616
    Purpose
    Bacterial expression plasmid for COVID-SARS2 NSP10 fused to GST
    Depositor
    Opher Gileadi
    Insert
    Covid-SARS2 Nsp10
    Use
    Tags
    GST-3C protease site
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159616-plasmid-map-sequence-id-311067

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  33. RFXANK (3UXG)

    Plasmid
    #36902
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK (RFXANK Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pKG194

    Plasmid
    #69757
    Purpose
    Mammalian Expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and eGFP
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. HaloTag-G protein gamma-10

    Plasmid
    #67041
    Purpose
    G protein gamma-10 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-10 (GNG10 Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67041-plasmid-map-sequence-id-115584

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  36. GYG1

    Plasmid
    #39091
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GYG1 (GYS1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. FL-DYSF-pUC57

    Plasmid
    #60177
    Purpose
    Plasmid contains full-length human dysferlin in a multiple cloning site of pUC57-Kan and can be easily cloned into other vectors.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60177-plasmid-map-sequence-id-96257

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  38. PS-CFP2-Alpha-5-Integrin-12

    Plasmid
    #62794
    Purpose
    Localization: Focal Adhesions, Excitation:400 / 490, Emission:468 / 511
    Depositor
    Michael Davidson
    Insert
    Alpha5Integrin (ITGAV Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62794-plasmid-map-sequence-id-210777

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  39. pLV Lnpk-V5

    Plasmid
    #175121
    Purpose
    Lentiviral expression of mouse Lnpk-V5
    Depositor
    Larry Gerace
    Insert
    Lnpk (Lnpk Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    175121-plasmid-map-sequence-id-351819

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  40. CNN3A-c002

    Plasmid
    #175456
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175456-plasmid-map-sequence-id-353174

    Enlarge

Showing: 4721 - 4760 of 8321 results