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pTubulin-miRFP670nano
Plasmid#127429PurposeHuman alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertmiRFP670nano-human alpha-tubulin
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58
Plasmid#171432PurposeExpression of hH3-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-hH3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP713-tubulin
Plasmid#136561PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP713DepositorInsertmiRFP713-tubulin
UseTagsmiRFP713ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS160 CRISPEY HDV-HDV NotI
Plasmid#114453PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsTagsExpressionYeastMutationPromoterScGAL7pAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Sam68 deltaKH
Plasmid#17688DepositorInsertSam68 (Khdrbs1 Mouse)
UseTagsHAExpressionMammalianMutationaa 157-256 deleted (KH domain)PromoterAvailable sinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP703-myosin
Plasmid#136565PurposeLC-Myosin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703-myosin
UseTagsemiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ALFA-tag-Gal4-VP16
Plasmid#199465PurposeExpression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.DepositorInsertGal4-ALFA-tag-VP16
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-NLS-dCas9-NLS
Plasmid#99908PurposeFor the expression of NLS-dCas9-NLSDepositorInsertNLS-dCas9-NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB
Plasmid#171427PurposeExpression of HaloTag N58-(GGS)X6-FRB in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F2
Plasmid#184167PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F1
Plasmid#184166PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLSU4cg10
Plasmid#126078PurposePlant transformation vector for stable expression of plastid targeted GFP1-10 in plant cellsDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterlongCaMV35SAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU3-gRNA
Plasmid#53063PurposeTranscription of guide RNA (gRNA) in rice cellsDepositorInsertOsU3 promoter + gRNA scaffold
UseCRISPR; Plant expressionTagsExpressionMutationPromoterrice U3Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJO629
Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58
Plasmid#171435PurposeExpression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInsertLifeact-(GGS)X6-HaloTag C58
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV132-vCas9
Plasmid#203223PurposeMammalian expression of vCas9DepositorInsertvCas9
UseAAV and CRISPRTagsExpressionMammalianMutationS55R R976A K1003A T1314RPromoterCBhAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-TEVp-IRES-mCherry
Plasmid#174377PurposeFor making TEVP cell line with hypBaseDepositorInsertTEV Protease
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only
