We narrowed to 16,113 results for: form

1. pET32a-hTGFa

   Plasmid

   #199236

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Transforming growth factor alpha, partial (TGFA Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p15aC-4D1D-5

   Plasmid

   #107866

   Purpose

   Bacterial expression of PIS, PI4K and PI4P5K

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Inserts

   PIS
   phosphatidylinositol 4-phosphate 5-kinase
   phosphatidylinositol 4-kinase β
   

   Tags

   myc

   Expression

   Bacterial

   Promoter

   proD and proD (in operon)

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-CLCa

   Plasmid

   #196912

   Purpose

   Visualization of clathrin-coated pit formation in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

   Insert

   Clathrin-Coated light chain
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2pA2-mnx1:MLS-KillerRed

   Plasmid

   #115515

   Purpose

   Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

   Depositor

   Marco Morsch

   Article

   Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

   Insert

   MLS-KillerRed
   

   Use

   Gateway cloning

   Tags

   MLS

   Promoter

   -3mnx1

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIP TBRII

   Plasmid

   #19147

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   Transforming growth factor, beta receptor II (TGFBR2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pYFP-NEAT1m_v1-RT

   Plasmid

   #97089

   Purpose

   Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1m_v1-RT (NEAT1 Human)
   

   Use

   CRISPR

   Tags

   EYFP

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYFP-NEAT1_IS_RT

   Plasmid

   #97091

   Purpose

   Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1_IS_RT (NEAT1 Human)
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 CARD8

   Plasmid

   #169962

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. L2-AA007

   Plasmid

   #185750

   Purpose

   Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

   Depositor

   Peter Szoevenyi

   Article

   Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

   Inserts

   HygR2
   eGFP
   

   Tags

   membrane localization tag Lti6b

   Expression

   Plant

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 TGFB1

    Plasmid

    #67080

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TGFB1 (TGFB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSFG-TFII-I-GFP beta

    Plasmid

    #22200

    Depositor

    Ananda Roy

    Article

    Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX2T merlin 340-595

    Plasmid

    #11634

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    merlin (NF2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Carboxy-terminal half of merlin isoform 1.

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

13. L2-AA012

    Plasmid

    #185751

    Purpose

    Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Inserts

    HygR2
    eGFP
    

    Tags

    membrane localization tag Lti6b

    Expression

    Plant

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 5mCUC1 pGreenII0129

    Plasmid

    #12067

    Depositor

    David Bartel

    Article

    Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

    Insert

    CUC1 mutant (CUC1 Mustard Weed)
    

    Use

    Plant transformation via agrobacterium

    Mutation

    miR-resistant CUC1. Made by introducing five sile…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

15. p15aC-3D1D-5

    Plasmid

    #121050

    Purpose

    Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
    

    Tags

    myc and none

    Expression

    Bacterial

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX2T merlin 340-590

    Plasmid

    #11633

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    merlin (NF2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Carboxy-terminal half of merlin isoform 2.

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pENTR-GFP-WT1 +KTS N terminal HA

    Plasmid

    #217639

    Purpose

    Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 +KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-GFP-WT1 -KTS N terminal HA

    Plasmid

    #217641

    Purpose

    Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Mutation

    p.408_410delKTS

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPEE47

    Plasmid

    #139426

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPEE46

    Plasmid

    #139425

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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