We narrowed to 25,014 results for: lars

1. ecAT3.10

   Plasmid

   #107215

   Purpose

   Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

   Depositor

   Mathew Tantama

   Article

   Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

   Insert

   ecAT3.10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MS2_GFP

   Plasmid

   #61764

   Purpose

   Expresses MS2 coat protein fused to GFP

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   MS2 coat protein fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB501NL3F10H

   Plasmid

   #141286

   Purpose

   No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Type

   Empty backbone

   Use

   Luciferase; Gateway

   Tags

   FLAG (x3), HIS (x10), and NLUC

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEED-mScarlet3

   Plasmid

   #219640

   Purpose

   Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEED-ALFA:HA

   Plasmid

   #219646

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   ALFA:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEED-sfGFP:ALFA

   Plasmid

   #219642

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-TurboID-3MYC-natMX6 (pFB1434)

   Plasmid

   #126050

   Purpose

   For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

   Depositor

   François Bachand

   Article

   Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

   Insert

   TurboID
   

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEED-mGreenLantern

   Plasmid

   #219643

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NW30

    Plasmid

    #133444

    Purpose

    Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW30
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PARG-c002

    Plasmid

    #173107

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARG (PARG Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S448-T976

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSEED

    Plasmid

    #219658

    Purpose

    Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Empty SEED Cassette (3xP3-dsRed)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG79-attL1-pegRNA-attL2

    Plasmid

    #213050

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEED-mGreenLantern:ALFA

    Plasmid

    #219644

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern:ALFA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAPV-DsRed2-ER-5

    Plasmid

    #170570

    Purpose

    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Parp14-c012

    Plasmid

    #173099

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    W1003-A1190

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Parp14-c010

    Plasmid

    #173100

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    F1208-G1388

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LT1_34_sfpHluorin

    Plasmid

    #177707

    Purpose

    Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEF1-sfpHluorin
    

    Use

    Used as donor in genome integration after lineari…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPE4max-Blasticidin

    Plasmid

    #194905

    Purpose

    Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

    Depositor

    George Church

    Article

    Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

    Insert

    BSD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV-U6+27-Tornado-Squash

    Plasmid

    #177915

    Purpose

    Expresses Squash aptamer as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-Squash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge