We narrowed to 59,686 results for: plasmid
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Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorPublicationInsertH11-L2 TALEN
UseTALENExpressionMammalianAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiN
Plasmid#37444PurposeControl lentiviral plasmid containing FLAG-HA tagsDepositorPublicationInsertFLAG-HA
UseLentiviralTagsFlag and HAExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
fwYellow chromoprotein
Plasmid#117841PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coliDepositorInsertpromoter, RBS, fwYellow
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pV1093
Plasmid#111428PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1DepositorPublicationInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFV3-lacZ
Plasmid#92074PurposeThis is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.DepositorInsertlacZ
UseLacz expression plasmid, for run-off transcriptio…Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRL_SRSF2_P95H_mCherry
Plasmid#84023Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95HPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)
Plasmid#64217PurposeExpression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
hU6 promoter; BbsI sites for sgRNA
H1 promoter; BamHI site for shRNA
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh, H1, and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304-YAP1(S94A)
Plasmid#59145PurposeYAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factorsDepositorAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorPublicationInsertH11-R2 TALEN
UseTALENExpressionMammalianAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO-shBRCA1 #1
Plasmid#44594DepositorAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSADdeltaG-mCherry
Plasmid#32636DepositorPublicationInsertmCherry
UseRabies virusExpressionMammalianPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2
Plasmid#164510PurposeGreen glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.DepositorHas serviceAAV9Insert(Cyto)iGlucoSnFR-mRuby2
UseAAVMutationSynthetic ConstructPromoterSynapsinAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBR_attB(bxb)_lox
Plasmid#183762PurposeBxb1-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionMammalianAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FDP-P10B2-A75-RZ-URA3
Plasmid#130874PurposeIntegration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tailDepositorInsertmKate
UseExpression of mkate, leu2(q180*), and ura3 on ort…Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE60-RRF-His
Plasmid#124132PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-iRFP670
Plasmid#99377PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-iRFP670
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBZCas13b
Plasmid#89898PurposeBacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.DepositorInsertCas13b
ExpressionBacterialPromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CD8a
Plasmid#86050PurposeA mammalian expression plasmid encoding CD8a without tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD8a (CD8A Human)
ExpressionMammalianPromoterCMV, T7Available sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by