We narrowed to 407 results for: ta cloning vector
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Plasmid#192545PurposepENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codonDepositorInsertpoly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
UseGateway entry vectorTagsExpressionMutationchanged Glu 614 to GlnPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_111del
Plasmid#65659PurposeFLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced stop codon such as to produce deletion…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-RFP entry
Plasmid#192278PurposeEntry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.DepositorInsertRFP flanked by type V-M CRISPR repeat sequences
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23119Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAGExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 R2pH-LAMP1-3xFLAG
Plasmid#157941Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAG, mCherry, and pHluorinExpressionMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS2
Plasmid#59390PurposeDestination vector with RBPMS2 for stable cell line generationDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR™221_RBPMS2
Plasmid#59389PurposepDONR vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsExpressionMutationPromoterT7Available sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas-pML-1B_6xHis-Cas12m
Plasmid#192282PurposeMmCas12m fused to His-tag for protein purificationDepositorInsertMmCas12m
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD44P pGL3
Plasmid#19122DepositorInsertCD44 promoter (CD44 Human)
UseNon viral cloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-UltraCas12a-6xNLS
Plasmid#209023PurposeLentiviral vector expressing human codon-optimized UltraCas12a with 6xNLSDepositorInsertUltraCas12a-6xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationM537R/F870LPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only