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pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0030

Plasmid

#162312

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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YCe1707 HC_Kan_RFP-p13

Plasmid

#100623

Purpose

EMMA toolkit part-entry vector position 13

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCY 3120-04

Plasmid

#36238

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECitrine-13Myc-TRP1

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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YEplac181-SOH1

Plasmid

#8623

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

SOH1 (SOH1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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mCherry-Vav2

Plasmid

#129623

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

Vav2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoSNAP

Plasmid

#136623

Purpose

Plasmid encoding outer mitochondrial localization SNAP-Tag

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0248

Plasmid

#162313

Purpose

Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-sfGFP-HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0134

Plasmid

#162307

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, HiBiT-stop, frame1 (NNT-TCG)

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCY 3110-01

Plasmid

#36236

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

mEos2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mEos2-kanMX

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEPYC0CM0257

Plasmid

#162308

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, HiBiT-stop, frame2 (TTC-G)

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0281

Plasmid

#162302

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, [S-tag]-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCY 3090-02

Plasmid

#36231

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEpolylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mCherry-hphMX4

Available Since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0029

Plasmid

#162311

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0028

Plasmid

#162310

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, 9 aa linker, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGC200

Plasmid

#19623

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Ppro-1<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pVCS9

Plasmid

#231623

Purpose

Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.

Depositor

Hans Christopher Bernstein

Article

Chan et al bioRxiv 2024.07.20.604438

Insert

Pcym-VanR-mKate2-Pvan-CymR-sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pvan/Pcym

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pJJB1462

Plasmid

#218623

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1mut-siRNAres_E

Plasmid

#146238

Purpose

Insect Expression of DmSMG6-PINNOB1mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

DmSMG6-PINNOB1mut-siRNAres (Smg6 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1-siRNAres_E

Plasmid

#146239

Purpose

Insect Expression of DmSMG6-PINNOB1-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

DmSMG6-PINNOB1-siRNAres (Smg6 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSMG6-siRNAres-V5His6_E

Plasmid

#146230

Purpose

Insect Expression of DmSMG6-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

DmSMG6-siRNAres (Smg6 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-8 GFP(+6)-tag2

Plasmid

#194623

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(+6)-(GGSKKRKKR)2

Tags

GGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0332

Plasmid

#162314

Purpose

Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

SP6prom-EO1-sfGFP-HiBiT

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0329

Plasmid

#162315

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease-HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0027

Plasmid

#162309

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, 9 aa linker, superfolder GFP, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0549

Plasmid

#162300

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, SUMO-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0550

Plasmid

#162301

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-SUMO-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0539

Plasmid

#162306

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, TEV protease (S219V), no stop codon

Use

Synthetic Biology

Mutation

S219V, codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPMY0SP0002

Plasmid

#162304

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, 6xHis tag-[HRV 3C cleavage site]

Use

Synthetic Biology

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0551

Plasmid

#162303

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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