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pBAD-HisD-miRFP

Plasmid

#108410

Purpose

Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Bacterial

Promoter

araB

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-GFP-mHP1alpha

Plasmid

#181912

Purpose

Bacterial protein expression vector for GFP-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon2-KGC-EGFP-ER2

Plasmid

#108430

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon2-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delWGR-L713F

Plasmid

#173941

Purpose

Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delWGR-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 521-660; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 PTEN C124S

Plasmid

#237340

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

C124S

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(T)

Plasmid

#206143

Purpose

Expresses the rs27895-T allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Tags

myc

Mutation

G346 changed to D346

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMECP2-RFP

Plasmid

#181905

Purpose

Fluorescently tagged MECP2, RFP

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

MECP2 (Mecp2 Mouse)

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-ZSGreen1-mHP1alpha

Plasmid

#181913

Purpose

Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

ZSGreen1

Expression

Mammalian

Promoter

TRE3G

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-Med26-NTD- K74A K75A

Plasmid

#49252

Purpose

Mammalian expression of flag-tagged human Med26-NTD- K74A K75A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

K74A, K75A, aa 1-339

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only
pPHR-mCherry-hNCL

Plasmid

#181886

Purpose

Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hNCL (NCL Mustard Weed, Human)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1beta

Plasmid

#181901

Purpose

Fluorescently tagged HP1beta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1beta (Cbx1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-UAS-zArchon1-KGC-EGFP-ER2

Plasmid

#108427

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zArchon1-KGC-EGFP-ER2

Use

Zebrafish

Promoter

UAS

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Archon1-KGC-GFP-ER2

Plasmid

#115892

Purpose

AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Has Service

AAV8

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

Syn

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Archon1-KGC-EGFP-ER2

Plasmid

#108420

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2

Plasmid

#108422

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Has Service

AAV5

Insert

Archon1-KGC-EGFP-ER2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-alpha-Tubulin-19-C

Plasmid

#108413

Purpose

Alpha-tubulin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

alpha-tubulin b (Tuba1b Mouse)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-Vimentin-17-C

Plasmid

#108414

Purpose

Vimentin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Vimentin (VIM Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Archon1-KGC-EGFP-ER2

Plasmid

#108419

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Archon2-KGC-EGFP-ER2

Plasmid

#108421

Purpose

AAV production plasmid encoding for Archon2 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-UAS-zArchon2-KGC-EGFP-ER2

Plasmid

#108428

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zArchon2-KGC-EGFP-ER2

Use

Zebrafish

Promoter

UAS

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-H2B-C1

Plasmid

#108411

Purpose

Histone 2B fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP-H2B (H2BC21 Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha

Plasmid

#181895

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha-delCTE

Plasmid

#181896

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha-delCTE (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-FUSN-mHP1alpha

Plasmid

#181897

Purpose

Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

FUSN-mHP1alpha (Cbx5 Mouse, Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-PGL-1-mHP1alpha

Plasmid

#181899

Purpose

Fluorescently tagged HP1alpha fused to PGL-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PGL-1-mHP1alpha (Cbx5 Nematode, Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGG-GFP-RGG-mHP1alpha

Plasmid

#181898

Purpose

Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

RGG-GFP-RGG (Cbx5 Nematode, Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPHR-mCherry-hNPM

Plasmid

#181887

Purpose

Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hNPM (NPM1 Mustard Weed, Human)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hHP1gamma

Plasmid

#181885

Purpose

Soluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hHP1gamma (CBX3 Mustard Weed, Human)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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