We narrowed to 329 results for: 10B

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pDEST47-ARL8A

Plasmid

#67466

Purpose

Mammalian expression of hArl8 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL8A-V5-His6

Plasmid

#67445

Purpose

Mammalian expression of hArl8 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pACYC-mTET1-N

Plasmid

#81056

Purpose

bacterial expression of N-terminus of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

N-terminus, amino acids 301-1366

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL8A-no stop

Plasmid

#67333

Purpose

hArl8a without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Plasmid

#200793

Purpose

Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

1-914; Δ505-610

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

Plasmid

#183411

Purpose

piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

Tet-on 3G

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ14256

Plasmid

#239275

Purpose

Expresses Kif5a*-PYL1 for the anterograde transport of RNA along microtubules via CRISPR-TO in primary mouse cortical neurons

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

Kif5a(aa1-559)-BFP-PYL1 (Kif5a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s pc3901

Plasmid

#201700

Purpose

Expresses of GFP-tagged mTet1s in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet1 CD Mut

Plasmid

#72218

Purpose

Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain (cata…

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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PEX3*-SBP-GFP

Plasmid

#120174

Purpose

Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Peroxisomal biogenesis factor 3 (PEX3 Human)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PEX3

Plasmid

#227303

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PEX3 (PEX3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

Plasmid

#134611

Purpose

Mammalian expression of mouse KIF5A 378-1027

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5A (Kif5a Mouse)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001147768)

Plasmid

#76194

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001147701)

Plasmid

#76191

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001145915)

Plasmid

#76192

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pmcherry-mTet1s pc3905

Plasmid

#201703

Purpose

Expresses of mcherry-tagged mTet1s in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001145643)

Plasmid

#76193

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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