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pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Use

Tags

(His)6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pAB064d

Plasmid

#79197

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ppro1

Available since

Dec. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMH-macroHC

Plasmid

#82337

Purpose

Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

Depositor

Michael Hothorn

Article

Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

Insert

SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)

Use

Tags

6xHis non-cleavable and macro domain of human his…

Expression

Bacterial

Mutation

Truncated protein, amino acids 1-178

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAB035a

Plasmid

#79190

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

Dec. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAB029f

Plasmid

#79220

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-SH2ABL1; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAB060c

Plasmid

#79196

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 luxAB; Ppro1 lambda cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ppro1

Available since

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD

Plasmid

#235574

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET_MBP-RGG-RGG

Plasmid

#124946

Purpose

Expression of MBP-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG

Plasmid

#124941

Purpose

Expresion of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

RGG domain

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_RGG-RGG-RGG

Plasmid

#124938

Purpose

Expression of RGG-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG

Plasmid

#124930

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAB076i3

Plasmid

#79198

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ppro1

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAB078d8

Plasmid

#79206

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1+2); Ppro1

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_RGG-GFP-RGG

Plasmid

#124948

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

EGFP and His

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAB078d3

Plasmid

#79203

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ppro1

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET_SZ1-RGG-RGG

Plasmid

#124942

Purpose

Expression of SZ1-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB092a

Plasmid

#79217

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1+2); Ppro1

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB078d9

Plasmid

#79207

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge