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pRDAX-VALENTIA-010 LifeAct-Neptune//BsaI

Plasmid

#130191

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Neptune

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-027 LifeAct-mVenus//BsaI

Plasmid

#130208

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-mVenus

Use

Synthetic Biology

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-009 LifeAct-mVenus//BsaI

Plasmid

#130190

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-mVenus

Use

Synthetic Biology

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-008 LifeAct-Eos//BsaI

Plasmid

#130189

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Eos

Use

Synthetic Biology

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-001 LifeAct-Sirius//BsaI

Plasmid

#130182

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Sirius

Use

Synthetic Biology

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

Plasmid

#175261

Purpose

labelling of microtubule plus end and F-actin structures

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)

Tags

mCherry (on iLifeact) and mNeonGreen (on EB3)

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

Plasmid

#229826

Purpose

Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet-I

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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GIL-6

Plasmid

#213389

Purpose

Expresses a chimeric cytokine between IL-6 and LIF

Depositor

Jurgen Scheller

Article

Rafii et al J Biol Chem. 2024 May;300(5):107251. doi

Insert

GIL-6

Tags

IgG1-Fc-tag and Myc-tg

Expression

Mammalian

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Tags

TwinStrep-tag

Expression

Mammalian

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Ttr

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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N1-G-Ca-FLITS

Plasmid

#191465

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDYH5

Plasmid

#52032

Purpose

Expression of GFP-tagged DAF-16 in C. elegans

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

DAF-16 (daf-16 Nematode)

Tags

GFP and HIS

Expression

Worm

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
4xmts-G-Ca-FLITS

Plasmid

#191462

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

4xmts

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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3xnls-G-Ca-FLITS

Plasmid

#191463

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

3xNLS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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ER-G-Ca-FLITS-KDEL

Plasmid

#191464

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

ER and KDEL

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTL-4

Plasmid

#52033

Purpose

Inducible expression of GST-unc43 in E. coli

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

Unc-43 (unc-43 Nematode)

Tags

GST

Expression

Bacterial

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pGI3EM22C

Plasmid

#135488

Purpose

Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr LifeAct-tdTomato

Tags

LifeAct

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFPO-His-G-Ca-FLITS

Plasmid

#191455

Purpose

Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

His

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAP28

Plasmid

#194707

Purpose

Linker::3xFlag::mScarlet::HAtag::Linker

Depositor

Alexandre Paix

Article

Seleit et al Elife. 2021 Dec 6;10. pii: 75050. doi: 1

Insert

Linker::3xFlag::mScarlet::HAtag::Linker

Use

Synthetic Biology

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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