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MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

Plasmid

#153195

Purpose

encodes pAAV NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Plasmid

#182063

Purpose

Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

Depositor

Adam Cohen

Article

Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

Insert

QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_SpikeyGi2_kv2.1

Plasmid

#195313

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_Flex_syn_SpikeyGi2_kv2.1

Plasmid

#195314

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin promoter

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

Plasmid

#175177

Purpose

Cre-dependent GCaMP6s and mBeRFP expression

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

GCaMP6s

Use

AAV

Tags

P2A-mBeRFP

Expression

Mutation

Promoter

CAG

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_TFAM_mKate2

Plasmid

#167331

Purpose

Expresses mKate2 fused to full length TFAM in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2

Plasmid

#161753

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2(T/A)

Plasmid

#161754

Purpose

Negative-control mutant for ExRai-AKAR2 biosensor.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2(T/A)

Use

Tags

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmExRai-AKAR2

Plasmid

#161755

Purpose

Plasma membrane-targeted ExRai-AKAR2.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

Lyn-ExRai-AKAR2

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mitoExRai-AKAR2

Plasmid

#161757

Purpose

ExRai-AKAR2 targeted to the outer mitochondrial membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

DAKAP-ExRai-AKAR2

Use

Tags

N-terminal 30 amino acids from DAKAP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-erExRai-AKAR2

Plasmid

#161756

Purpose

ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

CYP450-ExRai-AKAR2

Use

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-hTCAB1-puroR

Plasmid

#167459

Purpose

3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

TCAB1 (WRAP53 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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CP3

Plasmid

#223335

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-miniTurboID-EGFP-3HA

Use

Tags

miniTurboID-EGFP-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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CP1

Plasmid

#223333

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-TurboID-EGFP-3HA

Use

Tags

TurboID-EGFP-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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iSKetSnFR2

Plasmid

#140467

Purpose

Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

Depositor

Henry Lester

Article

Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

Insert

iSKetSnFR2

Use

Tags

His Tag, HA Tag and Myc tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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iSKetSnFR1

Plasmid

#140466

Purpose

Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

Depositor

Henry Lester

Article

Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

Insert

iSKetSnFR1

Use

Tags

His Tag, HA Tag and Myc tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hGfap-R-CaMP1.07-WPRE-SV40

Plasmid

#164140

Purpose

AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

Depositor

Hirokazu Hirai, Hajime Hirase

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

R-CaMP1.07

Use

AAV

Tags

Expression

Mutation

Promoter

hGFAP

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P1-LoxP-Neo-N-miRFP670-C

Plasmid

#159434

Purpose

CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

Depositor

Jianhua Xing

Article

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Inserts

Neomycin
miRFP670

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Expression

Mutation

Promoter

SV40

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_smFP Myc

Plasmid

#59757

Purpose

encodes multiple copies of an epitope tag Myc

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _Myc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge