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Addgene

We narrowed to 342 results for: mpg

Showing: 221 - 240 of 342 results
  1. AYBEv0.1

    Plasmid
    #193966
    Purpose
    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, EF1a, CBh
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pVH666

    Plasmid
    #169610
    Purpose
    Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).
    Depositor
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pVH664

    Plasmid
    #169608
    Purpose
    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).
    Depositor
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pVH663

    Plasmid
    #169607
    Purpose
    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).
    Depositor
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pIRGAL43C

    Plasmid
    #218278
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pIRGAL4A

    Plasmid
    #218277
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction
    Depositor
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pIRGAL3C

    Plasmid
    #218275
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pIRHDGAL43C

    Plasmid
    #218279
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pIRHDGAL3C

    Plasmid
    #218276
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 221 - 240 of 342 results