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We narrowed to 6,273 results for: nls

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Showing: 5951 - 6000 of 6273 results

  2. pH-ABE8e-spG

    Plasmid
    #200775
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9-spG(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200775-plasmid-map-sequence-id-399599

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  3. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid
    #52890
    Purpose
    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Inserts
    LexGAD
    Gal4 (GAL4 Budding Yeast)
    Expression
    Insect
    Available Since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52890-plasmid-map-sequence-id-211914

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  4. attB53-Pac-TRE-3xFlag-Neurog1-attB53

    Plasmid
    #183613
    Purpose
    RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Insert
    Neurog1 (Neurog1 Mouse)
    Use
    Rmce
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183613-plasmid-map-sequence-id-361602

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  7. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

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  8. Coexpressed VLP NES

    Plasmid
    #182433
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182433-plasmid-map-sequence-id-360116

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  9. Coexpressed VLP NES (M)

    Plasmid
    #182435
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182435-plasmid-map-sequence-id-360119

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  10. Coexpressed VLP NES (G)

    Plasmid
    #182477
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182477-plasmid-map-sequence-id-360429

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  16. pUC19-Q03JI6

    Plasmid
    #103123
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    Use
    CRISPR; Cloning vector
    Tags
    SV40NLS
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103123-plasmid-map-sequence-id-201885

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  17. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209044-plasmid-map-sequence-id-413636

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  18. pLenti-nSpCas9-TadA8.20m

    Plasmid
    #209045
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8.20m-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209045-plasmid-map-sequence-id-413618

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  20. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    112713-plasmid-map-sequence-id-217067

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  22. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

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  25. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209042-plasmid-map-sequence-id-413615

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  26. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  27. pLenti-nSpCas9-NoCBE

    Plasmid
    #209038
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209038-plasmid-map-sequence-id-413620

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  28. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  31. pcDNA4/TO-bio-myc-cCE

    Plasmid
    #112711
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Tags
    HIV Rev nuclear localization signal (NES), Stop c…
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV with Tet repressor binding sites
    Available Since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    112711-plasmid-map-sequence-id-217051

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  44. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid
    #210391
    Purpose
    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)
    Depositor
    Benjamin Kleinstiver
    Article
    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:
    Insert
    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    CamKII promoter for Cre, human U6 promoter for Sp…
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    210391-plasmid-map-sequence-id-415458

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Showing: 5951 - 6000 of 6273 results