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pHRIG-Akt3

Plasmid

#53596

Purpose

lentiviral expression of constitutively active human Akt3 and EGFP

Depositor

Heng Zhao

Article

Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

Insert

cAkt3 (AKT3 Human)

Use

Lentiviral

Tags

HA and IRES-EGFP

Expression

Mammalian

Mutation

constitutively active (myristoylated version)

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only
-204 human IFN-gamma luc

Plasmid

#17596

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-204 to +38 of promoter

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[Chronos-GFP]

Plasmid

#62722

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-BiP-Myc-KDEL T37G

Plasmid

#27165

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Mutation

ATP bound form: T37G

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
MSCV P2Gm FF

Plasmid

#19750

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pJG/ALPHA MHC

Plasmid

#55594

Purpose

transgene expression driven by ALPHA MHC promoter

Depositor

Jeffrey Robbins

Article

Gulick et al J Biol Chem. 1991 May 15;266(14):9180-5.

Type

Empty backbone

Expression

Mammalian

Promoter

alpha MHC

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCM5.47

Plasmid

#17254

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 3'utr in pDONRP2RP3

Use

Gateway Cloning

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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977 pSG5L HA PTEN A4

Plasmid

#10753

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

A4: S380A, T382A, T383A, S385A

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pCSF107mT-GATEWAY-3'-Myc tag

Plasmid

#67617

Purpose

A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Myc

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Insert

CDK8 (CDK8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pCM1.35

Plasmid

#17248

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

GFP:H2B from pKS111-His in pDONR201

Use

Gateway Cloning

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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T7-RelA(S276A)

Plasmid

#24153

Depositor

Warner Greene

Article

Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

Insert

RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

S276A

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
Pol

Plasmid

#65520

Purpose

Expresses HBV polymerase in mammalian cells

Depositor

Wang-Shick Ryu

Article

Ryu et al Virology. 2008 Mar 30;373(1):112-23. Epu

Insert

HBV polymerase protein

Tags

Flag

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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PX851

Plasmid

#62883

Purpose

N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX-VEcadTL

Plasmid

#45849

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

VE-cadherin (Cdh5 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PPP1R12A wt

Plasmid

#31658

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

Plasmid

#32139

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3RT> STOP STOP B3RT>

Expression

Insect

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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