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We narrowed to 63,680 results for: cloning

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Showing: 2381 - 2400 of 63680 results

  1. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  2. pAAVS1-P-CAG-GFPluc2

    Plasmid
    #80493
    Purpose
    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression
    Depositor
    Knut Woltjen
    Publication
    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFPluc2
    Use
    Donor vector (human)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80493-plasmid-map-sequence-id-151598

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  6. pEF5B-FRT-control-APEX

    Plasmid
    #73187
    Purpose
    Expresses "control"-APEX (cytosolic) in mammalian cells
    Depositor
    Maxence Nachury
    Publication
    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    control-APEX (Nphp3 Mouse, Glycine max)
    Tags
    internal GFP tag
    Expression
    Mammalian
    Mutation
    changed glycine 2 to alanine
    Promoter
    EF1alpha
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73187-plasmid-map-sequence-id-139537

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  8. pHL-EF1a-SphcCas9-iP-A

    Plasmid
    #60599
    Purpose
    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon usage optimized for human usage
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60599-plasmid-map-sequence-id-97109

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  10. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  17. CMV-Hylight-T152E

    Plasmid
    #193448
    Purpose
    A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)
    Depositor
    Richard Goodman
    Publication
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Binding-Dead Hylight
    Expression
    Mammalian
    Mutation
    Threonine 67 (ACT) mutated to a Glutamic Acid (GA…
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193448-plasmid-map-sequence-id-378448

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  19. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140005-plasmid-map-sequence-id-275488

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Showing: 2381 - 2400 of 63680 results