We narrowed to 24,243 results for: crispr

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pX330A_dCas9-1x6

Plasmid

#63600

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D1361Y

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138318

Purpose

Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SauCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x7

Plasmid

#63607

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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p426-SNR52p-gRNA.clr-2.Y-SUP4t

Plasmid

#89692

Purpose

gRNA for clr-2 locus

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gRNA for clr-2 locus

Use

CRISPR; Grna

Tags

No

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRU205

Plasmid

#167685

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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Mesp-1916/-1>nls::Cas9::nls

Plasmid

#59988

Purpose

Mesp driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Mesp -1916/-1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ158

Plasmid

#69307

Purpose

Gateway entry vector with hSpCas9 (Human codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9 (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x6

Plasmid

#63606

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_1

Plasmid

#135739

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_2

Plasmid

#135740

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCF820_U6-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138316

Purpose

Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-hsg(M)

Plasmid

#138262

Purpose

Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

sgRNA M

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x3

Plasmid

#63603

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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