We narrowed to 14,345 results for: TIM

Showing: 2421 - 2440 of 14345 results

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GeNL(Ca2+)_250/pcDNA3

Plasmid

#85206

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_250

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pFH45

Plasmid

#125810

Purpose

Level0 nSpCas9-ABE7.10 base changer, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nSpCas9-ABE7.10 base changer, human codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEB2-Katushka-9-5

Plasmid

#104011

Purpose

Plasmid encoding Katushka-9-5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka9-5

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (K6S, N21D, R32G, L…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-CaURA3

Plasmid

#44886

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-3.2mDelta13

Plasmid

#13560

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, S112A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pNB783

Plasmid

#60162

Purpose

Firefly luciferase (Promega) driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-CaURA3

Plasmid

#44879

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomWasabi-SpHis5

Plasmid

#44850

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomWasabi

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET10

Plasmid

#107440

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET10 (MET10 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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BCJJ344

Plasmid

#117503

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB-XIAP1-351

Plasmid

#11560

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 352-496

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pRSET FLII275Pglu-4.6m

Plasmid

#13565

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A G275S-ECFP-K276R

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby-SpHis5

Plasmid

#44843

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-CCK

Plasmid

#184602

Purpose

For expression of a fluorescent sensor for CCK in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-CCK

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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HIV-1 integrase F185H/C280S in pET15b

Plasmid

#61672

Purpose

HIV-1 integrase F185H/C280S in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

HIV-1 integrase F185H/C280S

Use

Synthetic Biology

Tags

His

Promoter

T7 promotor

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pEB1-Clover

Plasmid

#103991

Purpose

Plasmid encoding Clover

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Clover

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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