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pO-macs-ISH

Plasmid

#15856

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Olfactory specific medium-chain acyl CoA synthetase (Acsm4 Mouse)

Use

Cloning vector

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pcFN-C (chick fibronectin)

Plasmid

#14063

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Expression

Mammalian

Mutation

DraI - EcoRI fragment inserted in HincII-EcoRI si…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pTU2-A-RFP

Plasmid

#72954

Purpose

Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - A site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBW214J115-Amp32C

Plasmid

#78649

Purpose

pSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pSB4C5_J23101-CocE

Plasmid

#128129

Purpose

Expressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

CocE

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPromE8 (GB0914)

Plasmid

#68175

Purpose

Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBW211J109-Amp32C

Plasmid

#78646

Purpose

pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pT35S (GB0036)

Plasmid

#68187

Purpose

Provides the CaMV 35S terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Sncg

Plasmid

#105217

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

Insert

Sncg (Sncg Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEIIIB

Plasmid

#14060

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIB (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers were attached to HindIII - HinfI fr…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBW213J116-Amp32C

Plasmid

#78648

Purpose

pSB3K3 carrying J116-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J116-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pP2A11 (GB0082)

Plasmid

#68168

Purpose

Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pR-Avr

Plasmid

#22650

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1 (Myt1 Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
NSK163 CMV-TO-SMN2 Nluc - active (exon 6, 7, 8)

Plasmid

#247276

Purpose

Expresses human SMN2 exons 6, 7, 8 followed by Nluc

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SMN2 exons 6,7,8-Nluc (SMN2 Human)

Expression

Mammalian

Mutation

"A" insertion at position 49 of exon 7

Promoter

CMV-TO

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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SMT000

Plasmid

#216849

Purpose

Plasmid that encodes a sfGFP gene under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramphenicol resistant.

Depositor

Polly Fordyce

Article

Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2

Insert

Superfolder Green Fluorescent Protein

Use

Synthetic Biology

Expression

Bacterial

Mutation

S30R, Y39N, N105T, Y145F, I171V, and A206V

Promoter

T7

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2-3A

Plasmid

#238984

Purpose

In vitro transcription of mutant bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Celver et al J Biol Chem. 2002 Mar 15;277(11):9043-8.

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

I386A,V387A,F388A

Promoter

SP6

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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BtA1N

Plasmid

#238979

Purpose

In vitro transcription of bovine arrestin-1

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-1 (SAG Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2N

Plasmid

#238980

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pFP.R373

Plasmid

#138243

Purpose

Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GKA).SaP-dpol*.(CEL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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